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'protein synthesis' in keywords

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1	Author	Brigitte Kiefer, Laske	Requires cookie*
	Title	Jürgen
	Abstract	Protein synthesis after UV-and X-irradiation was investigated in diploid yeast. The incorpora tion of radioactively labelled lysine and phenylalanine was measured 2.5 and 4 hours after ex posure. By varying the specific activity the pool sizes could be estimated. At 2.5 hours there is some increase in pool sizes and a dose-dependent enhancement of protein synthesis. At 4 hours pools are again normal, but the increase of synthetic activity prevails.
	Reference	(Z. Naturforsch. 32c, 973 [1977]; received August 29 1977)
	Published	1977
	Keywords	Yeast, Protein Synthesis, Amino Acid Pools, Irradiation
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	TEI-XML for	default:Reihe_C/32/ZNC-1977-32c-0973.pdf
	Identifier	ZNC-1977-32c-0973
	Volume	32

2	Author	Joan Kraft-Creech, Ingrid Pietsch, Ernst-Randolf Lochmann	Requires cookie*
	Title	Über den G ehalt an m em brangebundenen und freien R ibosom en in Saccharom yceszellen nach Hem m ung der P roteinsynthese m it Cyclo- hexim id The Content of Membrane-Bound and Free R ibo somes in Saccharomyces after the Inhibition of P ro tein Synthesis with Cycloheximid
	Abstract	After the inhibition of protein synthesis in Saccharom yces cells with cycloheximid, the content of membrane-bound ribosomes decreases significantly, whereas the content of free ribosomes remains the same as the untreated control for a longer period of time. This is further evidence that in yeast cells protein synthesis takes place only on membrane-bound ribosomes.
	Reference	Z. Naturforsch. 33c, 299 (1978); eingegangen am 2. November 1977/1. Januar 1978
	Published	1978
	Keywords	Protein Synthesis, Cycloheximid, Membrane-Bound and Free Ribosomes
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	TEI-XML for	default:Reihe_C/33/ZNC-1978-33c-0299_n.pdf
	Identifier	ZNC-1978-33c-0299_n
	Volume	33

3	Author	C. Cocito, O. Tiboni, F. Vanlinden, O. Ciferri	Requires cookie*
	Title	Inhibition of Protein Synthesis in Chloroplasts from Plant Cells by Virginiamycin
	Abstract	The light-driven incorporation o f am ino acids by isolated spinach chloroplasts is inhibited by the M component (VM) and not by the S com ponent (VS) o f virginiamycin. This inhibitory effect is partially reversible. In chloroplast extracts, poly(U)-directed polyphenylalanine formation is strongly inhibited by VM and not by VS. The in vivo synergistic effect o f VM and VS observed in bacteria and algae, does not occur in isolated chloroplasts and chloroplast extracts.
	Reference	Z. Naturforsch. 34c, 1195—1198 (1979); received July 4 1979
	Published	1979
	Keywords	Chloroplasts, Protein Synthesis, A ntibiotics, Photosynthesis
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	TEI-XML for	default:Reihe_C/34/ZNC-1979-34c-1195.pdf
	Identifier	ZNC-1979-34c-1195
	Volume	34

4	Author	W. Olfram Koller, Helm Ut Kindl	Requires cookie*
	Title	Rates of de novo Synthesis of Malate Synthase and Albumins during the Very Early Phase of Germination
	Abstract	Malate synthase is synthesized de novo in the very early phase of germination. Its molecular and immunological properties do not differ from those o f malate synthase from fully developed cotyl edons. Radioactive leucine was administered to dry seeds of cucumber, and its incorporation into proteins o f cotyledons was examined after 2 days o f germination. The specific radioactivity of ma late synthase, purified by immunoprecipitation and electrophoresis on polyacrylamide gel, was only 1/20 the average value of the total albumin fraction. The minimal incorporation documented by the comparatively low specific activity o f isolated malate synthase is discussed in relation to the large pool of malate synthase already present in dry seeds.
	Reference	Z. Naturforsch. 34c, 1237 (1979); received July 23 1979
	Published	1979
	Keywords	Protein Synthesis, Albumins, Malate Synthase, Cucumis sativus
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	Identifier	ZNC-1979-34c-1237
	Volume	34

5	Author	Christian Kreutzfeldt	Requires cookie*
	Title	Initiation of Protein Synthesis in Yeast: Binding of Met-tRNAj
	Abstract	Conditions for the binding of Met-tRNAj to 40 s ribosomal subunits and to proteins isolated out o f the yeast ribosomal KC1 wash were investigated. Sucrose density gradient experiments revealed that binding of Met-tRNAj to 40 s ribosomal subunits was catalyzed in a AUG and GTP dependent reaction. Binding of Met-tRNAi to proteins of the ribosomal KC1 wash as assayed by the Millipore filter technique was found to be independent of AUG, GTP and 40 s ribosomal subunits. Additions of GTP yielded only slight stimulation, whereas Mg2+ caused dissociation of complexes. It was concluded that these reactions were most likely catalyzed by initiation factor eIF-2 although stimulation by GTP did not occur.
	Reference	Z. Naturforsch. 36c, 142—148 (1981); received September 18 1980
	Published	1981
	Keywords	Protein Synthesis, Initiation, Initiator tRNA, Yeast
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	TEI-XML for	default:Reihe_C/36/ZNC-1981-36c-0142.pdf
	Identifier	ZNC-1981-36c-0142
	Volume	36

6	Author	M. H. Ecker, A.-M Dünger, G. Wachlin, F. Mach	Requires cookie*
	Title	Untersuchungen über die Regulation der Proteinsynthese auswachsender Sporen von Bacillus subtilis Studies on Protein Synthesis of Outgrowing Spores of Bacillus subtilis
	Abstract	Two-dimensional gel electrophoresis according to O'Farrell [6] was used to study synthesis of membrane bound proteins during outgrowth of Bacillus subtilis spores. Three major classes of membrane bound proteins were distinguished, depending on the time of onset and duration of their synthesis: la — a great amount of proteins typical of vegetative cells made throughout all stages of outgrowth and vegetative growth; lb — vegetative proteins synthesized during middle and later outgrowth stages as well as during vegetative growth (sequential activation); II — outgrowth-specific proteins synthesized during early outgrowth but not in vegetative cells. A similar classification was made for total proteins [9]. In a temperature-sensitive mutant defective in early outgrowth the sequential activation of vegetative genes (group lb) is especially inhibited under restrictive conditions, whereas the syn thesis of group Ia-proteins and outgrowth-specific proteins (II) could be detected during early outgrowth stages. We suggest that the expression of outgrowth genes, whose products might be found among the outgrowth-specific proteins, could be involved in the sequential reactivation of vegetative genes during outgrowth. The vegetative proteins of group la (continuous synthesis) are produced throughout growth and differentiation. On the other hand the synthesis of vegetative proteins, group lb , is switched off during sporulation and switched on during outgrowth. The control mechanisms of the expression of these two groups of vegetative genes during growth and differen tiation are discussed.
	Reference	Z. Naturforsch. 40c, 421 (1985); eingegangen am 25. Oktober 1984/21. Februar 1985
	Published	1985
	Keywords	Bacillus subtilis, Spore Outgrowth, Protein Synthesis, Membrane Proteins
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	TEI-XML for	default:Reihe_C/40/ZNC-1985-40c-0421.pdf
	Identifier	ZNC-1985-40c-0421
	Volume	40

7	Author	Klaus Scheller, Peter Karlson, Dietrich Bodenstein	Requires cookie*
	Title	Effects of Ecdysterone and the Juvenile Hormone Analogue Methoprene on Protein, R N A and D N A Synthesis in Wing Discs of Calliphora vicina
	Abstract	Adolf Butenandt zum 75. G ebu rtstag gew idm et The protein, RNA and DNA content of wing discs increase exponentially during the last larval instar. Biosynthesis of protein, RNA and DNA was studied by injecting labelled precursors into larvae or white prepupae and measuring the incorporation in the wing discs dissected out after an appropriate time. Protein synthesis is stimulated by ecdysterone, methoprene has no effect. Bio synthesis of rRNA is increased in wing discs of white prepupae after ecdysterone or methoprene injection. Methoprene inhibits the synthesis of mRNA, while ecdysterone has no clear-cut effect within the limits of our method (gel electrophoretic analysis). Ecdysterone and methoprene have no detectable influence on incorporation of thymidine into DNA, but the incorporation label from uridine into DNA is diminished; this effect may be due to changes in the precursor pool.
	Reference	Z. Naturforsch. 33c, 253 (1978); received March 9 1978
	Published	1978
	Keywords	Ecdysterone, Juvenile Hormone, Wing Discs, Protein Synthesis, RNA Synthesis, DNA Synthesis, C alliph ora vicina
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	TEI-XML for	default:Reihe_C/33/ZNC-1978-33c-0253.pdf
	Identifier	ZNC-1978-33c-0253
	Volume	33

8	Author	Joachim Bollmann, Klaus Hahlbrock	Requires cookie*
	Title	Timing of Changes in Protein Synthesis Pattern in Elicitor-Treated Cell Suspension Cultures of Parsley ( Petroselinum crispum)
	Abstract	The timing o f changes in the protein synthesis pattern o f elicitor-treated, [35S]methionine-labelled parsley cells (Petroselinum crispum) was analyzed by two-dimensional gel electropho resis. Five groups were distinguished from a large number o f elicitor-responsive as well as un responsive proteins. Two groups were synthesized de novo either early or late after elicitor ap plication; two other groups were strongly reduced in their rates o f synthesis either early or late after elicitor application; and one group was not appreciably affected at all. The elicitor-in-duced changes altered the total protein com position considerably. A few selected, induced pro teins were functionally identified. These included two early induced enzymes, phenylalanine amm onia-lyase (PAL) and 4-coum arate:C oA ligase (4C L), and a late induced enzyme, a ber-gaptol O-methyltransferase (BM T) which is specifically involved in the biosynthesis o f furano-coumarin phytoalexins. The biological significance o f the observed differential timing o f changes in protein synthesis rates is discussed.
	Reference	Z. Naturforsch. 45c, 1011 (1990); received May 30 1990
	Published	1990
	Keywords	Cell Culture, Elicitor, Petroselinum crispum, Protein Synthesis, Two-Dim ensional Gel Electrophoresis
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	TEI-XML for	default:Reihe_C/45/ZNC-1990-45c-1011.pdf
	Identifier	ZNC-1990-45c-1011
	Volume	45

9	Author	B. M. Aršálek, M. Šimek, R. J. Smith	Requires cookie*
	Title	The Effect of Ecdysterone on the Cyanobacterium Nostoc 6720
	Abstract	The insect hormone ecdysterone stimulated dinitrogen fixation, heterocyst formation, cell size and protein synthesis in suspension cultures of the cyanobacterium Nostoc 6720 within 48 h when added to the culture at concentrations between 10-9 and 10-7 M . In the presence of the hormone the volume of vegetative cells increased by 6-fold and that of heterocysts by near ly twice as compared to untreated controls. All the observed effects were inhibited by the cal-cium-calmodulin inhibitor, trifluoperazine. Cholesterol used as a null control had no signifi cant effect.
	Reference	Z. Naturforsch. 47c, 726—730 (1992); received May 26/July 27 1992
	Published	1992
	Keywords	Cyanobacterium, Nostoc PCC 6720, Ecdysterone, Enhanced Nitrogen Fixation, Cell Size, Protein Synthesis
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	TEI-XML for	default:Reihe_C/47/ZNC-1992-47c-0726.pdf
	Identifier	ZNC-1992-47c-0726
	Volume	47

10	Author	A. Kiinnea, E. Pistoriusb, K. Kloppstechc, E. De, G. Rootd	Requires cookie*
	Title	Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control
	Abstract	Two proteins with apparent molecular masses of 17 and 24 kD that are synthesized in a circadian manner in the phytoflagellate Euglena gracilis, were recognized as proteins belong ing to the family of light-harvesting-chlorophyll-proteins (LHCPs) of class 1(17 kD) and of class II (24 kD). Identification was achieved by N-terminal sequencing of the proteins iso lated from two-dimensional polyacrylamide gels and by detection with an anti-LHCP II se rum. While it was found that the total am ount of LHCPs remains almost constant, when Euglena is grown under diurnal conditions (12 h light and 12 h dark), we could show that the amount of newly synthesized 17 and 24 kD proteins varies about 20-fold with a maximum of synthesis in the light phase. In contrast, the analysis of the mRNA levels at different times revealed only minor differences in the stationary concentration of the LHCP specific mRNA, indicating that the control of LHCP synthesis is at the translational level. Principally, the same finding was obtained using inhibitors of transcription. Thus, it is concluded that the expression of LHCPs in Euglena gracilis in contrast to that of higher plants is primarily regulated at the translational level.
	Reference	Z. Naturforsch. 53c, 1017—1026 (1998); received July 7/August 3 1998
	Published	1998
	Keywords	Euglena gracilis, Circadian Rhythm, Translational Control, Protein Synthesis, Light-Harvesting-Chlorophyll-Proteins
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	TEI-XML for	default:Reihe_C/53/ZNC-1998-53c-1017.pdf
	Identifier	ZNC-1998-53c-1017
	Volume	53

11	Author	Regina Teschner, Stefan Postius, Monika Löffler, Friedhelm Schneider	Requires cookie*
	Title	Proliferation and Metabolie Activities of Ehrlich Ascites Tumor Cells in Chemically Defined Albumin Media
	Abstract	, amino acid transport, respiration and lactate/glucose quotient of Ehrlich ascites tumor cells grown in suspension culture in serum free medium supplemented with albumin charges of different origin were studied. Optimal cell growth was obtained in nutrient medium supplemented with 1% bovine serum albumin (Cohn-fraction V, Serva). Cell proliferation under these culture conditions was delayed to 50% as compar ed to controls in normal medium; the rate of synthesis of macromolecules was reduced; energy metabolism was not significantly impaired. The trend of the cells in albumin medium to attach to glass was independent from the pH of the cultures between 7.2 and 8.0; it was enhanced by fatty acid deprivation of the albumin.
	Reference	Z. Naturforsch. 34c, 805 (1979); received May 16 1979
	Published	1979
	Keywords	Cultured Cells, Chemically Defined Albumin Media, Cell Proliferation Cell proliferation, viability, DNA-, RNA-, protein synthesis
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	TEI-XML for	default:Reihe_C/34/ZNC-1979-34c-0805.pdf
	Identifier	ZNC-1979-34c-0805
	Volume	34