| 1 | Author | Julia Yanevaa, Jordanka Zlatanova30 | Requires cookie* |
| Title | The Electrophoretic Separation of Curved Cisplatin-Modifled DNA Fragments on Polyacrylamide Gels Is Dependent on the Voltage Gradient  | ||
| Abstract | Polyacrylamide gel electrophoresis has been widely used to study DNA fragments containing sequence-de-pendent curvature. The anomalous electrophoretic be havior of curved DNA fragments on such gels allows their separation from straight fragments of the same length. H ere we dem onstrate that polyacrylamide gels can be successfully used to resolve DNA fragments m od ified at a single site by the antitum or drug ds-diam-m inedichloroplatinum (II) (ds-DDP, cisplatin) from their unmodified counterparts. However, the resolution strongly depends on the voltage gradient, being com pletely lost when it drops below a certain threshold level. The param eters of the electric field do not affect separation of 'norm al' DNA fragments of comparable length. | ||
| Reference | Z. Naturforsch. 53c, 921—923 (1998); received April 27/May 12 1998 | ||
| Published | 1998 | ||
| Keywords | Cisplatin, Curved DNA, Electric Field, Electrophoresis, Polyacrylamide Gels | ||
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