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1983[X]
1Author    Britta Verhoek, RenateKäthe Haas, Michael Wrage, Linscheid, Ernst HeinzRequires cookie*
 Title    Lipids and Enzymatic Activities in Vacuolar Membranes Isolated via Protoplasts from Oat Primary Leaves  
 Abstract    Vacuoles were released from oat (Avena sativa) mesophyll protoplasts and purified by sed i­ mentation and flotation. Disruption o f isolated vacuoles follow ed by density gradient centri­ fugation gave two m embrane bands which after com bination were further purified on sucrose gradients. A significant contam ination by microbodies, thylakoids, m itochondria, endoplasm ic reticulum and Golgi membranes can be excluded, whereas markers for plasma m em brane and chloroplast envelope were present in the final membrane preparation. In the purified membrane fraction the follow ing enzym atic activities were detected: N A D H -cytochrome C reductase E.C. 1.6.2.2, ATPase E.C. 3.6.1.3, UD PG : sterol glucosyltransferase, UDP-Gal: diacylglycerol galactosyltransferase E.C. 2.4.1.46, glucan synthetase II, C D P-choline: diacylglycerol phosphocholinetransferase E.C. 2.7.8.2, formation o f acylgalactosyl diacylglycerol and acyl-CoA thioesterase E.C. 3.1.2.2. N on e o f these can be considered to be sp ecific for the tonoplast. Acid phosphatase E.C. 3.1.3.2 was present in the cell sap. The vacuolar membranes contain phospholipids and glycolipids o f the most com plex com posi­ tion found so far in a membrane system isolated from m esophyll protoplasts. About h alf o f the glycolipids were accounted for by glycosyl diacylglycerols usually considered to be confined to plastids. Steryl glycosides and acyl steryl glycosides were other prominent glycolipids. A cere-broside was the predominating lipid com ponent o f this membrane preparation. 
  Reference    Z. Naturforsch. 38c, 770—7 (1983); received May 5 1983 
  Published    1983 
  Keywords    Contamination, Microsomal Enzymes, G alactolipids, Steryl G lycosides, Cerebrosides 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0770.pdf 
 Identifier    ZNC-1983-38c-0770 
 Volume    38