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1980 (1)
1979 (1)
1Author    Joseph Veser, H. Elm, Ut ThomasRequires cookie*
 Title    Immunological Studies of Catechol Methyltransferase from the Yeast Candida tropicalis  
 Abstract    Immunization o f rabbits with purified catechol methyltransferase from Candida tropicalis yielded a potent antiserum. Ouchterlony double diffusion analysis showed a single precipitin line. There was no cross reactivity with the catechol methyltransferase from rat and bovine liver. Specific antigen-antibody interaction was demonstrated by a potent inhibitory effect of the antibody on the yeast enzyme. Immunological titration and quantitative precipitin reaction of the enzyme showed that the maximum amount of precipitable complex occurred at the equivalence point where enzyme activity was completely inhibited. 
  Reference    Z. Naturforsch. 35c, 712—7 (1980); received April 24/May 27 1980 
  Published    1980 
  Keywords    Catechol Methyltransferase, Antibodies, Yeast Fungus, Candida tropicalis CBS 94 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0712.pdf 
 Identifier    ZNC-1980-35c-0712 
 Volume    35 
2Author    Joseph Veser, P. Eter, G.Requires cookie*
 Title    Purification and Properties of a Catechol Methyltransferase of the Yeast Candida tropicalis  
 Abstract    In an effort to investigate catechol methyltransferase activity in sources other than mam m alian tissues and cells, a high level o f enzyme activity was found in the yeast fungus Candida tropicalis CBS 94. Partial purification o f the enzyme (approx. 550 fold with a recovery o f 7%) could be achieved by using ion-exchange and gel filtration techniques. The molecular w eight was estimated at 32,000 ± 2,000 by gel filtration on Sephadex G-100. In isoelectric focusing experiments on Sephadex G-75 the enzym e exhibited a pl-value o f 5.0 ± 0.1. In contrast to catechol methyltransfer­ ase from various m am m alian tissues the enzyme activity was prepared from the pH 5-sediment. The substrate specifity is com parable to other catechol methyltransferases. 
  Reference    Z. Naturforsch. 34c, 709 (1979); received April 24 1979 
  Published    1979 
  Keywords    Catechol Methyltransferase, Yeast Fungus, Candida tropicalis CBS 94, Enzyme Purification, Catechols 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0709.pdf 
 Identifier    ZNC-1979-34c-0709 
 Volume    34