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1Author    N. Yckowski, S. S. BrodyRequires cookie*
 Title    Interactions of Monomolecular Films of Retinal at Alkaline pH  
 Abstract    The surface properties of mixed monomolecular films of retinal and phospholipids were studied at a nitrogen/water interface. The subphase was glycine buffer pH 10.5 with an ionic strength of 0.1. Monomolecular films of retinal in the presence of amino acids were also measured. The area per molecule, at a surface pressure of 10 dyn/cm, A 10, in the dark for 9-cis retinal and 11-ci's retinal are 42 A 2 and 47 A 2, respectively. After irradiation A 10 for 9-cis retinal and 11-cts retinal dcerease to 40 A 2 and 43 A2, respectively. The surface potentials, AV, at a surface pressure of 10 dyn/cm, in the dark for 9-cis retinal and 11-cis retinal are 470 mV and 445 mV, respectively. After irradiation, AV for 9-cis retinal decreases to 435 mV and 11-cis retinal increases to 490 mV. Interaction was observed between retinal and phospholipids and amino acids. The A 10 and AV 10 of mixed films of retinal and phospholipid were measured as a function of the mole fraction of phospholipid. Maximum interaction is observed at mole ratios of; phosphatidylserine/9-ct's = 1; phosphatidylethanolamine/9-m = 2, phosphatidylserine/ll-cis = 3; phosphatidylethanolamine/ H-CJ5 = 3. It is shown that mixing and interaction between phosphatidylethanolamine and retinal is spontaneous. The A 10 and A V 10 of films of retinal were measured as a function of the molar concentration of amino acid in the subphase. The nature of the interaction between retinal and phospholipid and amino acid are discussed. 
  Reference    (Z. Naturforsch. 29c, 327 [1974]; received April 1 1974) 
  Published    1974 
  Keywords    ) Vision, Retinal, Monolayers, Membranes, Model systems 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0327.pdf 
 Identifier    ZNC-1974-29c-0327 
 Volume    29