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1992 (2)
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1Author    J.M F G Aerts, A. W. SchramRequires cookie*
 Title    Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals I. A Comparison of Isolation Procedures  
 Abstract    Vacuoles were isolated from the upper epiderm is o f petals o f Petunia hybrida line R27 using three different procedures. U tilizing the vacuolar localization o f anthocyanin, vacuolar prep­ arations obtained through polybase treatment, osm otic shock and m echanical forces were com ­ pared on purity, yield and stability. The com parison indicated that application o f the polybase procedure results in the best vacuolar preparations. Vacuoles could be obtained which m ain­ tained their acidic pH and retained their anthocyanin content. 
  Reference    Z. Naturforsch. 40c, 189—1 (1985); received June 26/O ctober 26 1984 
  Published    1985 
  Keywords    Petunia hybrida, Vacuoles, Protoplasts, Anthocyanins 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0189.pdf 
 Identifier    ZNC-1985-40c-0189 
 Volume    40 
2Author    J.M F G Aerts, A. W. SchramRequires cookie*
 Title    Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals ü . Vacuolar Localization of Some Hydrolases  
 Abstract    In vacuoles isolated from petal upper epiderm is o f Petunia hybrida through polybase-induced lysis o f protoplasts the vacuolar localization o f x-m annosidase and x-galactosidase was dem on­ strated. N o association was observed between the tonoplast and these hydrolases. Protoplasts from different mutant lines blocked in anthocyanin biosynthesis were treated with DEAE-dextran to release vacuoles. The differences in quality o f the obtained vacuolar preparations indicated the relation between mutant line used and successful application o f the polybase-procedure. 
  Reference    Z. Naturforsch. 40c, 196 (1985); received June 26/O ctober 26 1984 
  Published    1985 
  Keywords    Petunia hybrida, Vacuoles, Anthocyanins, Flavonoids, H ydrolases 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0196.pdf 
 Identifier    ZNC-1985-40c-0196 
 Volume    40 
3Author    Ulrike Mackenbrock, Ralph Vogelsang, Wolfgang BarzRequires cookie*
 Title    Isoflavone and Pterocarpan Malonylglucosides and ß -l ,3 -G l u c a n -and Chitin-Hydrolases are Vacuolar Constituents in Chickpea (Cicer arietinum L.)  
 Abstract    Cell suspension cultures of chickpea (Cicer arietinum L.) were used to prepare protoplasts and vacuoles. The vacuolar preparation revealed only slight contaminations of cytoplasmic marker enzymes. H PLC analysis of the vacuolar extract showed that the malonylglucosides of isoflavones, isoflavanones and pterocarpans are exclusively located in the vacuole. Experi­ ments designed to determine the subcellular localization of the isoflavone malonylglucoside: malonylesterase suggest an association of this enzyme with the vacuolar membrane. Finally, a ß-l,3-glucanase and a chitinase with basic isoelectric points were also found to be localized in the chickpea vacuoles. 
  Reference    Z. Naturforsch. 47c, 815—822 (1992); received August 17 1992 
  Published    1992 
  Keywords    Chickpea, Cicer arietinum, Cell Suspension Cultures, Protoplast, Vacuole 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0815.pdf 
 Identifier    ZNC-1992-47c-0815 
 Volume    47 
4Author    Florenz Sasse, Dietlinde Backs-Hüsemann, Wolfgang BarzRequires cookie*
 Title    Isolation and Characterization of Vacuoles from Cell Suspension Cultures of Daucus carota  
 Abstract    A reliable procedure for the isolation o f vacuoles from anthocyanin-containing cells o f Daucus carota cell suspension cultures has been developed. From cells o f the late linear growth phase, protoplasts were prepared and purified in a sucrose/sorbitol gradient. Vacuoles were liberated from these protoplasts by osm otic shock and purified in a M etrizamide step gradient. The vacuole-containing fractions were analysed for their anthocyanin content as a measure for the yield o f vac­ uoles. The purity o f vacuoles was examined by assaying various marker enzym es in both proto­ plasts and vacuoles. The purest vacuolar fraction had 8% o f the total activity o f glucose-6-phos-phate dehydrogenase (marker for cytosol), 8% o f cytochrom e oxidase (m itochondria) and 10% o f N A DPH -cytochrom e c reductase (ER). 55% o f the acid phosphatase activity o f the protoplasts and 35% o f the total malate were recovered in the vacuoles. The vacuolar pool o f am ino acids is quite large. D ata for 15 am ino acids show that 44 to 73% are being located in the vacuole. 
  Reference    Z. Naturforsch. 34c, 848—853 (1979); received M ay 15 1979 
  Published    1979 
  Keywords    Daucus carota, Protoplasts, Vacuoles, Lysosomes, A m ino Acids, Anthocyanin 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0848.pdf 
 Identifier    ZNC-1979-34c-0848 
 Volume    34 
5Author    Friedrich, C. Hristian, B. Aum AnnRequires cookie*
 Title    A Patch Clamp Study of Tonoplast Electrical Properties in Vacuoles Isolated from Chenopodium rubrum Suspension Cells  
 Abstract    -W ilhelm B en tru p , B ernd H offm ann , M aria G o g arten -B o ek els, Jo h a n n P ete r G ogarten The patch clamp technique has been applied to isolated vacuoles from green suspension cells of Chenopodium rubrum to record electrical parameters of the tonoplast. In a symmetrical K* solution of 46 mM, the membrane displays a near zero voltage, whereas 2 mM ATP will hyper-polarize it to 15 or 20 mV (vacuole positive). The conductance amounts to about one S-m~2. Fluctuations of the clamp current are explained by an unknown channel species having opening times of 5 — 10 ms. Together with previous work on a tonoplast vesicle preparation and unpub­ lished data on vacuoles from our laboratory, the present results indicate an electrogenic mem­ brane ATPase pumping protons from the cytoplasm to the vacuole. 
  Reference    Z. Naturforsch. 40c, 886 (1985); received October 23 1985 
  Published    1985 
  Keywords    Patch Clamp, Membrane ATPase, Tonoplast, Vacuole, Chenopodium rubrum L 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0886.pdf 
 Identifier    ZNC-1985-40c-0886 
 Volume    40 
6Author    M. Arie-Theres, Hauser, Michael WinkRequires cookie*
 Title    Uptake of Alkaloids by Latex Vesicles and Isolated Mesophyll Vacuoles of Chelidonium ntajus (Papaveraceae)  
 Abstract    Latex vacuoles o f Chelidonium majus (Papaveraceae) naturally sequester protoberberine and benzophenanthridine alkaloids. Isolated vesicles take up a number o f other heterologous alkaloids, such as 9,10-dihydroergocryptine, vinblastine, strychnine, nicotine, colchicine, lupanine, and 13-hydroxylupanine, with a strong preference for the indole alkaloids and for nicotine. Kinetic data clearly show that the uptake proceeds via simple diffusion and not by carrier-mediated transport. The alkaloids are concentrated in the vesicles against a concentra­ tion gradient. M gA TP has no influence on alkaloid uptake. It is likely that alkaloids are trapped in the vesicles by chelidonic acid (concentration in latex 58 mM, in vesicles 661 mM) which com plexes these alkaloids and thus prevents their rediffusion out o f the vesicles. Uptake o f these alkaloids by leaf protoplasts and vacuoles o f Ch. majus, by vacuoles o f suspension-cultured cells o f Lupinuspolyphyllus and by rabbit erythrocytes was studied for comparison. 
  Reference    Z. Naturforsch. 45c, 949 (1990); received January 15/May 18 1990 
  Published    1990 
  Keywords    Chelidonium m ajus, Alkaloid Uptake, Vacuole, Latex, Diffusion, Ion-Trap 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0949.pdf 
 Identifier    ZNC-1990-45c-0949 
 Volume    45 
7Author    R. Schmitt, H. SandermRequires cookie*
 Title    Specific Localization of /J-D-Glucoside Conjugates of 2,4-Dichlorophenoxyacetic Acid in Soybean Vacuoles  
  Reference    Z. Naturforsch. 37c, 772—777 (1982); received May 17 1982 
  Published    1982 
  Keywords    2, 4-Dichlorophenoxyacetic Acid, Glycine m ax L, Plant Cell Suspension Culture, Vacuole, /?-ü-Glucosides 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0772.pdf 
 Identifier    ZNC-1982-37c-0772 
 Volume    37 
8Author    A. Ntonio, A. Calderón, E. G. Arcía-Florenciano, Marí, A. Pedreño, Rom Ualdo, M. Uñ Oz, A.Ros BarcelóRequires cookie*
 Title    The Vacuolar Localization of Grapevine Peroxidase Isoenzymes Capable of Oxidizing 4-Hydroxystilbenes  
 Abstract    A study o f the subcellular localization o f peroxidase isoenzymes capable o f oxidizing 4-hydroxystilbenes to viniferin-type com pounds has been carried out in cultured cells derived from Vitis vinifera cv. G am ay berries. The study revealed that these isoenzym es are apparently located, in soluble form, in the vacuolar sap and are strongly inhibited by vacuolar anthocya-ni(di)ns in the concentration range found in planta. These results suggest that the inhibition o f the 4-H S peroxidase system in vacuoles, as a consequence o f anthocyani(di)n accum ulation, could be responsible for the loss o f viniferin production potential which accompanies grape berry veraison. 
  Reference    Z. Naturforsch. 47c, 215 (1992); received July 24/N ovem ber 14 1991 
  Published    1992 
  Keywords    Anthocyani(di)ns, Peroxidase Isoenzymes, Vacuole, Viniferin Synthesis, Vitis vinifera cv Gam ay 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0215.pdf 
 Identifier    ZNC-1992-47c-0215 
 Volume    47