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1Author    Hans HarmsRequires cookie*
 Title    Uptake and Conversion of Three Different 5-Ring Polycyclic Aromatic Hydrocarbons (PAH s) in Cell Suspension Cultures of Various Chenopodiaceae-Species  
 Abstract    Three 14C-labelled 5-ring PAHs, benzo(a)pyrene, dibenz(a,h)anthracene and perylene were added to various cell suspension cultures o f plants belonging to the Chenopodiaceae family. The differently configurated 5-ring systems varied in uptake and metabolism. The uptake of benzo(a)pyrene was highest in all cell cultures tested whereas the assimilation of dibenz(a,h)an-thracene and perylene was significantly less. The uptake o f benzo(a)pyrene and dibenz(a,h)-anthracene was highest in those cultures which showed the highest increase in biomass. In contrast the uptake of perylene was highest in cultures whose increase in biomass was less. Previous investigations (H. Harms, W. Dehnen and W. Mönch, Z. Naturforsch. 32 c, 3 2 1 -3 2 6 (1977)) showed that benzo(a)pyrene is metabolized to quinones and other oxygenated derivatives by Chenopodium rubrum cell cultures. It was demonstrated now that in further reactions these compounds are incorporated into insoluble fractions. With increasing time o f incubation the amount of radioactivity in the extracted residue increased. The proportion o f oxygenated derivatives formed seems to be correlated with the incorporation of radioactivity into insoluble fractions. Dibenz(a,h)anthracene turned out to be the most stable 5-ring system whereas perylene is metabolized to a certain extent but only small amounts o f radioactivity could be detected in the extracted insoluble residue. The experiments showed that plant cell cultures maintain their specific behaviour over long periods. On the other hand each o f the chemical compounds, because of its molecular size and configuration, is subject to its own specific metabolism in plant systems. 
  Reference    Z. Naturforsch. 38c, 382—382 (1983); received December 30 1982/March 2 1983 
  Published    1983 
  Keywords    5-Ring Polycyclic Aromatic Hydrocarbons, Uptake, Metabolism, Plant Cell Suspension Cultures, Chenopodiaceae 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0382.pdf 
 Identifier    ZNC-1983-38c-0382 
 Volume    38 
2Author    Istvan JablonkaiRequires cookie*
 Title    Basis for Differential Chemical Selectivity of MG-191 Safener against Acetochlor and EPTC Injury to Maize  
 Abstract    The influence of MG-191 safener on the uptake, translocation and metabolism of [l4C]acetochlor and [14C]EPTC was studied. The amounts o f absorbed radioactivity by maize seedlings at 3, 6, 24, and 72 h after applications of [l4C]labeled herbicides and [l4C]MG-191 were different. Plants treated with [l4C]acetochlor took up 30-to 50-fold more radiolabel with­ in 72 h than [14C]EPTC-or [14C]MG-191-treated plants. Addition of MG-191 caused only mi­ nor changes in the rate of herbicide absorption. EPTC and MG-191 and/or their metabolites moved quickly acropetally and partitioned equally between root and shoot tissues up to 72 h. The amount of acetochlor and/or its labeled metabolites translocated to shoot tissues was less than 10%. MG-191 practically had no influence on herbicide translocation rates. With all chemicals the amounts of water-soluble and unextractable fractions increased while the ratio of hexane-extractable metabolites decreased with time. TLC analyses of both water-and hex­ ane-soluble metabolites confirmed the fast metabolism o f acetochlor. The acetochlor metabo­ lism took place via GSH conjugation and more polar, non-conjugated metabolites compared to parent molecule were detected in hexane-soluble fraction. MG-191 enhanced acetochlor metabolism by decreasing the portion of non-metabolized acetochlor. EPTC metabolism re­ sulted in water-soluble metabolites having similar chromatographic properties to those of ace­ tochlor. However, there was no safener effect on non-metabolized EPTC content of plants. It appears that MG-191 protects maize against EPTC by enhancing the early rate of conjugation with GSH after initial oxidative metabolism. 
  Reference    Z. Naturforsch. 46c, 836—845 (1991); received March 26 1991 
  Published    1991 
  Keywords    Chemical Selectivity, Safener, MG-191, Acetochlor, EPTC, Uptake, Translocation, Metabo­ lism 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0836.pdf 
 Identifier    ZNC-1991-46c-0836 
 Volume    46 
3Author    A. Kio Kobayashi1, JoK. Myong, Im, Kazuyoshi Kawazu0Requires cookie*
 Title    Uptake and Exudation of Phenolic Compounds by Wheat and Antimicrobial Components of the Root Exudate  
 Abstract    In the course of our study, it was found that phenyl propenoic acid derivatives were readily taken up by wheat. Leaf leachate components were chosen for the feeding experiments and /j-coumaric acid, ferulic acid and caffeic acid were found to be quickly taken up into the plants via the roots. The analytical study revealed that the exudate contained potent antim i­ crobial compounds together with amino acids and sugars. Besides the primary metabolites, 4-hydroxystyrene, 3-methoxy-4-hydroxystyrene and 3-methoxy-4-acetoxystyrene were identified as exudate components from wheat roots in sterile hydroponic culture. This indicates that these antimicrobial components may play a significant role in the defense system as allelochemicals for the rhizosphere. 
  Reference    Z. Naturforsch. 51c, 527—533 (1996); received January 31/ 
  Published    1996 
  Keywords    Decarboxylation, p-Coumaric Acid Ferulic Acid, Caffeic Acid, Uptake, Exudate, Antimicrobial Styrenes 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0527.pdf 
 Identifier    ZNC-1996-51c-0527 
 Volume    51