| | 1 | Author
| D. Ieter, S. Chubert, K. Arin Boss | Requires cookie* | | | Title
| Analytical Ultracentrifugation o f Proteins in Solutions o f Triton X-100 Using Absorption Optics  | | | | Abstract
| Analytical ultracentrifugation studies on proteins in solu tions of the nonionic detergent Triton X-100, by means of a UV scanning system, can be conveniently performed by the following procedure: (1) Scanning of the cells is done at a wavelength of 246 nm. (2) The sedimentation of the deter gent is prevented by appropriate adjustment of the solvent density. (3) The reference cell is filled with water. Reliable molecular weight determinations can be performed at de tergent concentrations up to 0.4% (w/w) in cells of path-length d = 12 mm and at correspondingly higher concen trations for d — 3 mm. Approx. 10 ng of protein are suffi cient for a determination. The method should be useful for studies of membrane proteins. | | | |
Reference
| Z. Naturforsch. 40c, 908—911 (1985); received June 20/ August 5 1985 | | | |
Published
| 1985 | | | |
Keywords
| Analytical Ultracentrifugation, UV Scanning System Proteins, Triton X-100 | | | |
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