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1984 (1)
1Author    RitaM. Fink, ErichF. ElstnerRequires cookie*
 Title    Comparison of Different Methods for the Determination of Phenylalanine Hydroxylase Activity in Rat Liver and Euglena gracilis  
 Abstract    Three different methods for the determination of phenylalanine hydroxylase activity have been compared: a) Differential photometric assay of the increase in tyrosine concentration in the presence of phenylalanine; b) Product separation by thin layer chromatography and scintillation counting of the [14C]tyrosine formed; c) HPLC separation and spectrofluorometric quantification of derivatized amino acids. A comparison of the activities of phenylalanine hydroxylase in rat liver and Euglena gracilis clearly showed that only rat liver contains this enzymic activity as shown by methods b) and c) although pseudo-activity of Euglena gracilis preparations was found during the spectrophoto-metric test a). The HPLC method proved to be the fastest, most reliable and convenient method for direct tyrosine determination and thus for measuring phenylalanine hydroxylase activity. 
  Reference    Z. Naturforsch. 39c, 728—733 (1984); received January 27/April 14 1984 
  Published    1984 
  Keywords    Phenylalanine Hydroxylase, Rat Liver, Euglena gracilis, Tyrosine Determination 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0728.pdf 
 Identifier    ZNC-1984-39c-0728 
 Volume    39