| | 1 | Author
| Heiner Strasser, Christina Hoffmann, Hans Grisebach, Ulrich Matern | Requires cookie* | | | Title
| Are Polyphosphoinositides Involved in Signal Transduction of Elicitor-Induced Phytoalexin Synthesis in Cultured Plant Cells?  | | | | Abstract
| The phospholipids of cultured parsley and soybean cells were labelled with myo-[2-3H]inositol, [2-3H]glycerol or [32P]orthophosphate. By one-and two-dimensional chromatographic compari son of the labelled phospholipids with reference substances, the presence of l-(3-sn-phos-phatidyl)-D-myo-inositol 4-phosphate and l-(3-sn-phosphatidyl)-D-myo-inositol 4,5-bisphosphate was demonstrated in these cultures. These results were corroborated by analysis of the deacyla-tion products. Cells were labelled with either myo-[2-3H]inositol, [2-3H]glycerol or [32P]ortho-phosphate and subsequently challenged with elicitor for various lengths of time. Radioactivity in individual phosphoinositides from these cells was determined. No significant influence of elicitor-challenge of either soybean or parsley cells on incorporation of 3H or 32P into polyphospho inositides was found between 0.5 and 20 min after elicitor addition. Intro d u ctio n | | | |
Reference
| Z. Naturforsch. 41c, 717 (1986); received April 17/May 7 1986 | | | |
Published
| 1986 | | | |
Keywords
| Polyphosphoinositides, Elicitors, Parsley (Petroselinum crispum) Cells, Soybean (Glycine max) Cells, Turnover | | | |
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| default:Reihe_C/41/ZNC-1986-41c-0717.pdf | | | | Identifier
| ZNC-1986-41c-0717 | | | | Volume
| 41 | |
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