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'Tulipa' in keywords Facet   Publication Year 1981  [X]
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1981[X]
1Author    Rainer Sütfeld, Rolf WiermannRequires cookie*
 Title    Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals  
 Abstract    Chalcone synthase was isolated from both anthers of Tulipa cv. "A peldoorn" and petals of Cosmos sulphureus Cav. After certain prepurification steps, the enzymes were further purified using gel chrom atography on Sephadex G-200 followed by repeated hydroxylapatite absorption chromatography. Both the enzymes showed the same chrom atographic properties. After gel chrom atography as well as after the first hydroxylapatite fractionation, the reaction products appeared as flavanones. However, after the second hydroxylapatite step, production of chalcones was observed. Like the enzyme from tulip anthers, the synthase from Cosmos petals produced the correspondingly substituted chalcones when p-coumaroyl-CoA, caffeoyl-CoA and feruloyl-CoA, respectively, were used as substractes. In both the cases, the ratios o f the different chalcones produced were found to be about the same. The appearance o f chalcone synthesis in this in vitro assay is caused by the complete elim ination of chalcone isomerase in the purification procedure. The importance of the isomerase for flavonoid biosynthesis, particularly in plant systems which are accumulating chalcones, is discussed. 
  Reference    Z. Naturforsch. 36c, 30—3 (1981); received N ovem ber 12 1980 
  Published    1981 
  Keywords    Tulipa, Cosmos, Biosynthesis, Flavonoids, Chalcone Synthase 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0030.pdf 
 Identifier    ZNC-1981-36c-0030 
 Volume    36 
2Author    Dieter Strack, Gesine Sachs, Axel Römer, Rolf WiRequires cookie*
 Title    Ferulic Acid-Sucrose Esters from Immature Anthers of Tulipa cv. "Apeldoorn"  
 Abstract    Two ferulic acid conjugates have been isolated from the extract of immature anthers of Tulipa cv. "Apeldoorn" and their structures elucidated using chromatography, electron impact mass spectrometry, and NMR spectroscopy. These two conjugates are 6,3'-diferuloylsucrose and 6,3',4'-triferuloylsucrose. High performance liquid chromatographic analysis of the extract showed that these esters are transient major constituents of the phenylpropanoid pattern of the immature anthers and are present in the ratio of 1:2. Previous investigations showed that these components are accumulated in the loculus. 
  Reference    Z. Naturforsch. 36c, 721—723 (1981); received April 30 1981 
  Published    1981 
  Keywords    Tulipa, Liliaceae, Hydroxycinnamic Acid Conjugates, Ferulic Acid Esters, Sucrose 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0721.pdf 
 Identifier    ZNC-1981-36c-0721 
 Volume    36