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'Triazine Resistance' in keywords Facet   Publication Year 1990  [X]
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1Author    Dirk Naber, Udo Johanningmeier, JackJ S Van Rensen, D. N., J.J S V RRequires cookie*
 Title    A Rapid Method for Partial mRNA and DNA Sequence Analysis of the Photosystem II psbA Gene  
 Abstract    Single amino acid substitutions in the D 1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between hel­ ices IV and V of the protein. The increasing number of herbicide-resistant organisms necessi­ tates development of a rapid methodology to characterize deviations from the wildtype se­ quence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D 1. These procedures involve the isolation and amplification of D N A and RN A and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-re-sistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D 1 protein, is found in the resistant plant. 
  Reference    Z. Naturforsch. 45c, 418—422 (1990); received November 3 1989 
  Published    1990 
  Keywords    Triazine Resistance, Sequence Analysis, Polymerase Chain Reaction, Chenopodium album 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0418.pdf 
 Identifier    ZNC-1990-45c-0418 
 Volume    45 
2Author    R. Hain, J. E. ThomzikRequires cookie*
 Title    Segregation of Triazine-Resistant Chloroplasts after Introduction into Winter Type Oilseed Rape (Brassica napus L.) by Protoplast Fusion  
 Abstract    Triazine-resistant chloroplasts o f the Canadian spring oilseed rape variety OAC Triton were transferred into four German winter oilseed rape lines and two cultivars o f double-low quality by means o f protoplast fusion. X-irradiation has been used to reduce the am ount o f nuclear D N A o f the spring type cultivar and to promote cybrid formation. RFLP-analysis showed that some regenerants and their progeny carried both types o f chloroplasts. In som e instances regenerants and progeny containing mixtures o f both chloroplasts not kept under selective conditions lost their triazine-resistant chloroplasts completely during further plant growth. Preliminary results o f greenhouse and field experiments indicate that volunteer plants can be eliminated by application o f 1 5 0 -3 0 0 g/ha metribuzin (SencorR, Bayer A G) in a stand o f triazine-resistant oilseed rape o f double-low quality. 
  Reference    Z. Naturforsch. 45c, 478 (1990); received September 8 1989 
  Published    1990 
  Keywords    Brassica napus, Somatic Hybridization, Triazine resistance, Chloroplast segregation, RFLP-analysis 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0478.pdf 
 Identifier    ZNC-1990-45c-0478 
 Volume    45 
3Author    MarcelA K Jansen, Klaus PfisterRequires cookie*
 Title    Conserved Kinetics at the Reducing Side of Reaction-Center II in Photosynthetic Organisms; Changed Kinetics in Triazine-Resistant Weeds  
 Abstract    The decay o f chlorophyll variable fluorescence after a "single turnover" flash is generally assumed to represent the reoxidation o f the reduced quinone Qa. We have observed that the kinetics o f this decay are very similar in a wide variety o f species. Comparing 28 different spe­ cies, we found an average half decay time o f 314 ± 4 6 (isec. N o systematic correlations were found between the decay rate and biochemical or physiological specializations such as C 2, C 4 or C A M . This indicates that structural as well as functional factors controlling photosystem II electron transfer between Qa and Qb are highly conserved. Apparently, the freedom for natural structural variations in this region is very limited. Triazine resistant plants, characterized by an altered amino acid sequence o f the D 1 protein, have clearly decreased rates o f Qa/Q b electron transfer. We found an average half decay time o f 946 ± 100 jisec (5 species). However, this three-fold decrease is much less than previously re­ ported. Therefore, if alterations o f photosystem II electron transfer efficiency contributes to an often reported reduction o f "ecological fitness", this contribution is smaller than was hitherto assumed. 
  Reference    Z. Naturforsch. 45c, 441—4 (1990); received November 27 1989 
  Published    1990 
  Keywords    Chlorophyll Fluorescence, Evolution, Photosystem II, Triazine Resistance, p sb A Conservation 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0441.pdf 
 Identifier    ZNC-1990-45c-0441 
 Volume    45