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1Author    Akira TaketoRequires cookie*
 Title    Sensitivity of Escherichia coli to Viral Nucleic Acid. XVI. Temperature Conditions for Ca2+-Dependent DNA Uptake in Escherichia coli  
 Abstract    Properties of C a2+-or Ba2+-dependent transfection and transform ation in Escherichia coli were examined, using (PX174 replicative-form (R F) D NA and plasm id DNA. F or the transfection and transformation, a heat pulse step was dispensable and the yield o f transfectants was, in most E. coli strains, rather reduced by the heat treatment. The heat pulse step was also detrim ental for the transformation o f certain strains such as lipopolysaccharide mutants. The first stage o f the DNA uptake process (formation of DNA • recipient cell complex) was dependent on low tem perature and Ca2+ ion. A substantial am ount o f the complexed R F-D N A was released from the bacteria, by washing with a chilled Tris buffer. Although a R F-D N A • cell complex was formed even at 37 °C or in chilled 0.05 m MgCl2, the complex did not yield transfectants. 
  Reference    Z. Naturforsch. 37c, 87—92 (1982); received October 141981 
  Published    1982 
  Keywords    DNA, Transfection, Transformation, Escherichia coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0087.pdf 
 Identifier    ZNC-1982-37c-0087 
 Volume    37 
2Author    Dagmar Busmann, RalfG. Ünter BergerRequires cookie*
 Title    Oxyfunctionalization o f a-and ß-Pinene by Selected Basidiomycetes  
 Abstract    Several strains of basidiom ycetes were examined for their ability to transform a-and ß-pinene in agitated submerged cultures. Four major metabolites of a-pinene (verbenol, ver-benone, myrtenol, and rrorcs-pinocarveol) and three main metabolites of ß-pinene (1,4-cineol, myrtenol, and rrarcs-pinocarveol) were isolated from the fermentation broth. The metabolic pathways included allylic oxidation, oxidative cleavage and further regioselective oxidation. G anoderm a applanatum was found to carry out the stereoselective allylic hydroxylation of a-pinene to verbenol, and o f ß-pinene to trans-pinocarveol in trans position to the C -C bridge. The optimal conditions of the bioreaction were established with respect to substrate concentration, incubation time and conversion time. D ue to growth inhibition caused by elevated substrate concentration, the bioconversion of ß-pinene required pre-grown cultures. Generally, mycelial pellet cultures were supplemented with the terpene substrate when a residual glucose content of 50% was reached. Depending on strain this point was reached after about 48 h. A n incubation period of two to three days gave best yields. The transient accumulation of oxygenated products apparently reflected different reaction velocities of the successive catabolic steps. 
  Reference    Z. Naturforsch. 49c, 545 (1994); received May 26/June 20 1994 
  Published    1994 
  Keywords    Pinene, Cineol, Basidiomycetes, Bioconversion, Transformation 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0545.pdf 
 Identifier    ZNC-1994-49c-0545 
 Volume    49 
3Author    Thomas GrafRequires cookie*
 Abstract    An in vitro transformation of bone marrow cells has been demonstrated for two strains of avian erythroblastosis virus (AEV-R and AEV-ES4). The transformed cells were indistinguishable from in vivo transformed erythroblasts in morphology and staining characteristics and could be pro­ pagated to large numbers. The transformation efficiency could be greatly increased by the addition of dimethyl-sulfoxide (DMSO). The number of foci appearing in the presence of DMSO was proportional to the virus concen­ tration. 
  Reference    (Z. Naturforsch. 30c, 847 [1975]; received October 10 1975) 
  Published    1975 
  Keywords    Avian Erythroblastosis Virus, Avian Hemopoietic Cells, Transformation, Erythropoietin, Dimethylsulfoxide 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0847_n.pdf 
 Identifier    ZNC-1975-30c-0847_n 
 Volume    30 
4Author    J. Berlinab, C. Rügenhagena, M. Rippert, S. ErdoganRequires cookie*
 Title    Effects of Culture Conditions on Isoflavonoid Levels of Transformed and Non-Transformed Cultures of Lupinus -a Comparison of Suspension and Hairy Root Cultures  
 Abstract    Some highly productive suspension and hairy root cultures were found among several trans­ formed cultures o f Lupinus polyphyllus and L. hartwegii. A transformed suspension culture Lupo 30150 and a root culture Luha 15834 containing the highest specific isoflavone glucoside content were characterized and compared with normal phytohormone-dependent lines with respect to product stability as well as to their responsiveness to external triggers, e.g. response to changes in the medium. While phytohorm one-dependent suspension cultures lost their ini­ tial ability to form increased levels o f isoflavonoids on phytohormone-free medium, the trans­ formed phytohorm one-independent suspension Lupo 30150 remained a highly productive line, despite the fact that its specific levels decreased to 60% o f the initial values during several years in liquid medium. Highest stability o f product patterns and levels were noted for the transformed root culture. Phytohorm ones had little effect on growth and isoflavonoid levels in suspension cultures, while they reduced both strongly in root cultures. In the presence o f 2,4-D the root culture changed into an aggregated low producing suspension culture from which the root state was recovered on phytohorm one-free medium. As long as the root state was main­ tained, isoflavonoid levels could not be distinctly improved by media variation while specific isoflavonoid levels o f suspensions were increased by stress factors such as phosphate depletion. When suspensions were transferred to fresh medium phenylalanine ammonia-lyase was greatly induced within 24 h, while the activity remained nearly unchanged in root cultures. 
  Reference    Z. Naturforsch. 46c, 735—7 (1991); received April 23 1991 
  Published    1991 
  Keywords    Lupinuspolyphyllus, Lupinus hartwegii, Transformation, Hairy R oot Cultures, Isoflavonoids 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0735.pdf 
 Identifier    ZNC-1991-46c-0735 
 Volume    46 
5Author    Halina Wysokińska, Katarzyna Lisowska, Katarzyna Floryanowicz-CzekalskaRequires cookie*
 Title    Transformation of Catalpa ovata by Agrobacterium rhizogenes and Phenylethanoid Glycosides Production in Transformed Root Cultures  
 Abstract    Transformed root cultures of Catalpa ovata were established following shoots infection with four agropine strains of A grobacterium rhizogenes. Frequency of root formation was dependent on the bacterial strain and the presence o f acetosyringone in the incubation m e­ dium. It is the first report concerning the possibility o f transforming Catalpa ovata by A. rhizogenes. Both transformed and untransformed root cultures o f C. ovata were studied for their growth and phenylethanoid glycoside production. A s with the roots of intact plants, cis-and trans-verbascoside as well as martynoside were produced in transformed and untrans­ formed root cultures of C. ovata. In hairy roots, total (cis + trans) verbascoside production could be stimulated up to three-fold of that of roots o f 6 -month-old plants grown in a green­ house, by using an appropriate root line cultured in liquid Zi B5 Gamborg medium containing indole-3-butyric acid (0.1 mg/1) in the dark but not light conditions. Transformed and untrans­ formed root cultures of C. ovata were also found to have 10 times higher martynoside pro­ duction than roots of intact plants. 
  Reference    Z. Naturforsch. 56c, 375—3 (2001); received D ecem ber 28 2000/February 13 2001 
  Published    2001 
  Keywords    Catalpa ovata, Transformation, Phenylethanoid G lycosides 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-0375.pdf 
 Identifier    ZNC-2001-56c-0375 
 Volume    56 
6Author    H. Schäfer, K. Taraz, H. BudzikiewiczRequires cookie*
 Title    Zur Genese der amidisch an den Chromophor von Pyoverdinen gebundenen Dicarbonsäuren |1] On the Genesis of the Dicarboxylic Acids Bound Amidically to the C hrom ophor of the Pyoverdins [1]  
 Abstract    Pseudomonas strains o f the so-called fluorescent group usually produce several pyoverdins which differ only in the nature o f a dicarboxylic acid bound amidically to the chromophor. For the pyoverdins isolated from the culture medium o f Pseudomonas fluorescens 12 it is shown that succinic acid is an artefact formed by hydrolysis o f succinic amide, and that a-ketoglutaric acid is transformed enzymatically to glutamic acid. This process is reversed after the phase o f exponential growth o f the bacteria. The ratio C4-vs. C 5-acids changes with the culture time and with increasing Fe3+ content o f the medium in favor o f the latter. 
  Reference    Z. Naturforsch. 46c, 398 (1991); received December 21 1990/March 13 1991 
  Published    1991 
  Keywords    *Pyoverdins, Biogenesis, Transformations, Citric Acid Cycle, Pseudomonas fluorescens 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0398.pdf 
 Identifier    ZNC-1991-46c-0398 
 Volume    46 
7Author    J. Berlinab, I. N. Kuzovkinac, C. Rügenhagenab, L. Fecker3, U. C. Om, M. Andeur3, V. W. RaybRequires cookie*
 Title    Hairy Root Cultures of Peganum harmala II. Characterization of Cell Lines and Effect of Culture Conditions on the Accumulation of ß-Carboline Alkaloids and Serotonin  
 Abstract    Hairy root cultures o f Peganum harmala established by genetic transformation with A gro­ bacterium rhizogenes wild type strains A 4, 15834 or carrying the binary vector pLTCgus 1, re­ spectively, were analyzed with respect to their tryptophan-derived m etabolites. Beside the pre­ viously detected ß-carboline alkaloids harmine, harmol, harmaline and harmalol all hairy root cultures were found to contain the ß-carboline glucoside ruine and serotonin. The appearance o f the root cultures and the levels o f ß-carboline alkaloids were greatly affected by the nitrogen source o f the medium. In the presence o f higher levels o f ammonium ions (as in MS medium) the roots were short and thick, while in B50 medium (low ammonium) they were thin and long. Ammonium ions had a negative effect on the levels o f ß-carbolines. The best root cul­ tures accumulated on B50 1 .5 -2 % ß-carbolines on a dry mass basis and in the presence o f 6% sucrose ca. 10 mg ß-carbolines/L x d were formed. In the presence o f phytohorm ones root cul­ tures were converted into low producing suspension cultures, from which the highly produc­ tive roots were reinduced on phytohormone-free medium. 
  Reference    Z. Naturforsch. 47c, 222 (1992); received July 29/Novem ber 11 1991 
  Published    1992 
  Keywords    Peganum harmala, R oot Cultures, Transformation, ß-Carboline Alkaloids, Serotonin 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0222.pdf 
 Identifier    ZNC-1992-47c-0222 
 Volume    47