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'Transformation' in keywords Facet   Publication Year 2001  [X]
Facet   section ZfN Section C:Volume 056  [X]
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1Author    Halina Wysokińska, Katarzyna Lisowska, Katarzyna Floryanowicz-CzekalskaRequires cookie*
 Title    Transformation of Catalpa ovata by Agrobacterium rhizogenes and Phenylethanoid Glycosides Production in Transformed Root Cultures  
 Abstract    Transformed root cultures of Catalpa ovata were established following shoots infection with four agropine strains of A grobacterium rhizogenes. Frequency of root formation was dependent on the bacterial strain and the presence o f acetosyringone in the incubation m e­ dium. It is the first report concerning the possibility o f transforming Catalpa ovata by A. rhizogenes. Both transformed and untransformed root cultures o f C. ovata were studied for their growth and phenylethanoid glycoside production. A s with the roots of intact plants, cis-and trans-verbascoside as well as martynoside were produced in transformed and untrans­ formed root cultures of C. ovata. In hairy roots, total (cis + trans) verbascoside production could be stimulated up to three-fold of that of roots o f 6 -month-old plants grown in a green­ house, by using an appropriate root line cultured in liquid Zi B5 Gamborg medium containing indole-3-butyric acid (0.1 mg/1) in the dark but not light conditions. Transformed and untrans­ formed root cultures of C. ovata were also found to have 10 times higher martynoside pro­ duction than roots of intact plants. 
  Reference    Z. Naturforsch. 56c, 375—3 (2001); received D ecem ber 28 2000/February 13 2001 
  Published    2001 
  Keywords    Catalpa ovata, Transformation, Phenylethanoid G lycosides 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-0375.pdf 
 Identifier    ZNC-2001-56c-0375 
 Volume    56