ZfN: Search Results

1	Author	J. Berlinab, C. Rügenhagena, M. Rippert, S. Erdogan	Requires cookie*
	Title	Effects of Culture Conditions on Isoflavonoid Levels of Transformed and Non-Transformed Cultures of Lupinus -a Comparison of Suspension and Hairy Root Cultures
	Abstract	Some highly productive suspension and hairy root cultures were found among several trans formed cultures o f Lupinus polyphyllus and L. hartwegii. A transformed suspension culture Lupo 30150 and a root culture Luha 15834 containing the highest specific isoflavone glucoside content were characterized and compared with normal phytohormone-dependent lines with respect to product stability as well as to their responsiveness to external triggers, e.g. response to changes in the medium. While phytohorm one-dependent suspension cultures lost their ini tial ability to form increased levels o f isoflavonoids on phytohormone-free medium, the trans formed phytohorm one-independent suspension Lupo 30150 remained a highly productive line, despite the fact that its specific levels decreased to 60% o f the initial values during several years in liquid medium. Highest stability o f product patterns and levels were noted for the transformed root culture. Phytohorm ones had little effect on growth and isoflavonoid levels in suspension cultures, while they reduced both strongly in root cultures. In the presence o f 2,4-D the root culture changed into an aggregated low producing suspension culture from which the root state was recovered on phytohorm one-free medium. As long as the root state was main tained, isoflavonoid levels could not be distinctly improved by media variation while specific isoflavonoid levels o f suspensions were increased by stress factors such as phosphate depletion. When suspensions were transferred to fresh medium phenylalanine ammonia-lyase was greatly induced within 24 h, while the activity remained nearly unchanged in root cultures.
	Reference	Z. Naturforsch. 46c, 735—7 (1991); received April 23 1991
	Published	1991
	Keywords	Lupinuspolyphyllus, Lupinus hartwegii, Transformation, Hairy R oot Cultures, Isoflavonoids
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	TEI-XML for	default:Reihe_C/46/ZNC-1991-46c-0735.pdf
	Identifier	ZNC-1991-46c-0735
	Volume	46

2	Author	H. Schäfer, K. Taraz, H. Budzikiewicz	Requires cookie*
	Title	Zur Genese der amidisch an den Chromophor von Pyoverdinen gebundenen Dicarbonsäuren \|1] On the Genesis of the Dicarboxylic Acids Bound Amidically to the C hrom ophor of the Pyoverdins [1]
	Abstract	Pseudomonas strains o f the so-called fluorescent group usually produce several pyoverdins which differ only in the nature o f a dicarboxylic acid bound amidically to the chromophor. For the pyoverdins isolated from the culture medium o f Pseudomonas fluorescens 12 it is shown that succinic acid is an artefact formed by hydrolysis o f succinic amide, and that a-ketoglutaric acid is transformed enzymatically to glutamic acid. This process is reversed after the phase o f exponential growth o f the bacteria. The ratio C4-vs. C 5-acids changes with the culture time and with increasing Fe3+ content o f the medium in favor o f the latter.
	Reference	Z. Naturforsch. 46c, 398 (1991); received December 21 1990/March 13 1991
	Published	1991
	Keywords	*Pyoverdins, Biogenesis, Transformations, Citric Acid Cycle, Pseudomonas fluorescens
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	TEI-XML for	default:Reihe_C/46/ZNC-1991-46c-0398.pdf
	Identifier	ZNC-1991-46c-0398
	Volume	46