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1982 (1)
1975 (1)
1Author    Akira TaketoRequires cookie*
 Title    Sensitivity of Escherichia coli to Viral Nucleic Acid. XVI. Temperature Conditions for Ca2+-Dependent DNA Uptake in Escherichia coli  
 Abstract    Properties of C a2+-or Ba2+-dependent transfection and transform ation in Escherichia coli were examined, using (PX174 replicative-form (R F) D NA and plasm id DNA. F or the transfection and transformation, a heat pulse step was dispensable and the yield o f transfectants was, in most E. coli strains, rather reduced by the heat treatment. The heat pulse step was also detrim ental for the transformation o f certain strains such as lipopolysaccharide mutants. The first stage o f the DNA uptake process (formation of DNA • recipient cell complex) was dependent on low tem perature and Ca2+ ion. A substantial am ount o f the complexed R F-D N A was released from the bacteria, by washing with a chilled Tris buffer. Although a R F-D N A • cell complex was formed even at 37 °C or in chilled 0.05 m MgCl2, the complex did not yield transfectants. 
  Reference    Z. Naturforsch. 37c, 87—92 (1982); received October 141981 
  Published    1982 
  Keywords    DNA, Transfection, Transformation, Escherichia coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0087.pdf 
 Identifier    ZNC-1982-37c-0087 
 Volume    37 
2Author    Akira TaketoRequires cookie*
 Title    Ba2+-Induced Competence for Transfecting D NA  
 Abstract    Effect of alkaline earth metal ions on induction of the competence for DNA transfection was investigated. Unlike spheroplasts, the bulk of the bacteria treated with these ions retains colony-forming ability. The order of effectiveness for transfection of replicative-form DNA has been found to be Ba2+> C a 2+> S r 2+> M g 2+. The competence of Ba2+-treated cells is 3 to 5 times higher than that of Ca^-treated bacteria and about 40 times higher than that of lysozyme-EDTA sphero­ plasts. The Ba^-dependent transfection is cryophilic and formation of the infective complex occurs very rapidly at 0 °C, but not at 37 °C. 
  Reference    (Z. Naturforsch. 30c, 520—522 [1975]; received March 11 1975) 
  Published    1975 
  Keywords    Ba2+, Competence, DNA, Escherichia coli, Transfection 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0520.pdf 
 Identifier    ZNC-1975-30c-0520 
 Volume    30