| | 1 | Author
| Akira Taketo | Requires cookie* | | | Title
| Sensitivity of Escherichia coli to Viral Nucleic Acid. XVI. Temperature Conditions for Ca2+-Dependent DNA Uptake in Escherichia coli  | | | | Abstract
| Properties of C a2+-or Ba2+-dependent transfection and transform ation in Escherichia coli were examined, using (PX174 replicative-form (R F) D NA and plasm id DNA. F or the transfection and transformation, a heat pulse step was dispensable and the yield o f transfectants was, in most E. coli strains, rather reduced by the heat treatment. The heat pulse step was also detrim ental for the transformation o f certain strains such as lipopolysaccharide mutants. The first stage o f the DNA uptake process (formation of DNA • recipient cell complex) was dependent on low tem perature and Ca2+ ion. A substantial am ount o f the complexed R F-D N A was released from the bacteria, by washing with a chilled Tris buffer. Although a R F-D N A • cell complex was formed even at 37 °C or in chilled 0.05 m MgCl2, the complex did not yield transfectants. | | | |
Reference
| Z. Naturforsch. 37c, 87—92 (1982); received October 141981 | | | |
Published
| 1982 | | | |
Keywords
| DNA, Transfection, Transformation, Escherichia coli | | | |
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| | | | | TEI-XML for
| default:Reihe_C/37/ZNC-1982-37c-0087.pdf | | | | Identifier
| ZNC-1982-37c-0087 | | | | Volume
| 37 | |
| 2 | Author
| Akira Taketo | Requires cookie* | | | Title
| Ba2+-Induced Competence for Transfecting D NA | | | | Abstract
| Effect of alkaline earth metal ions on induction of the competence for DNA transfection was investigated. Unlike spheroplasts, the bulk of the bacteria treated with these ions retains colony-forming ability. The order of effectiveness for transfection of replicative-form DNA has been found to be Ba2+> C a 2+> S r 2+> M g 2+. The competence of Ba2+-treated cells is 3 to 5 times higher than that of Ca^-treated bacteria and about 40 times higher than that of lysozyme-EDTA sphero plasts. The Ba^-dependent transfection is cryophilic and formation of the infective complex occurs very rapidly at 0 °C, but not at 37 °C. | | | |
Reference
| (Z. Naturforsch. 30c, 520—522 [1975]; received March 11 1975) | | | |
Published
| 1975 | | | |
Keywords
| Ba2+, Competence, DNA, Escherichia coli, Transfection | | | |
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| | | | | TEI-XML for
| default:Reihe_C/30/ZNC-1975-30c-0520.pdf | | | | Identifier
| ZNC-1975-30c-0520 | | | | Volume
| 30 | |
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