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1996 (1)
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1Author    Wilhelm Menke, GeorgH. SchmidRequires cookie*
 Title    Fluorescence and Photochemical Properties of Plants with Defective Photosystem II  
 Abstract    The mykotrophic orchid Neottia nidus-avis does not evolve oxygen in the light but is able to perform photophosphorylation. The low temperature fluorescence emission spectrum lacks the 680 and 690 nm bands. Hence, the spectroscopic chlorophyll a forms which are attributed to photosystem II do not occur in plastids of this orchid. The low temperature excitation spectrum of photosystem I fluorescence exhibits a maximum at 666 nm. The position o f this maximum ap­ pears not to be influenced by energy transfer and corresponds to the absorption maximum of the chlorophyll form which emits the photosystem I fluorescence. Energy migration, however, occurs from carotenoids whose absorption spectrum is shifted to longer wavelengths and which cause the yellow-brown color of the Neottia plastids. Room temperature fluorescence emission shows after the onset of light no variable part. Despite the fact that plastids of the tobacco mutant N C 95 at most evolve only traces o f oxygen the low temperature emission spectrum shows the three bands which are usually observed with fully functioning chloroplasts. However, the two bands at 680 and 690 nm are distinctly lower than with the wild type. The variable portion o f room temperature fluorescence is barely detectable. In line with the very low capacity for oxygen evolution, rates of electron transport partial reactions in the region of photosystem II are extremely low. In agreement with this observation no 690 nm absorption change signal is detected. However, a normal P7 + 00 signal is seen. In the presence of electron donors like reduced phenazine methosulfate the decay time o f the P7 ^0 signal is faster than with the wild type. The yellow tobacco mutant Su/su var. aurea which exhibits at high light intensities higher rates of photosynthesis than the wild type shows at low temperature an emission spectrum with stronger photosystem II bands than the wild type. 
  Reference    Z. Naturforsch. 35c, 461 (1980); received March 14 1980 
  Published    1980 
  Keywords    Photosystem II, Low Temperature Fluorescence, Tobacco, Neottia 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0461.pdf 
 Identifier    ZNC-1980-35c-0461 
 Volume    35 
2Author    Heinz Rennenberg, Reinhard Steinkamp, Andrea PolleRequires cookie*
 Title    Evidence for the Participation of a 5-Oxo-prolinase in Degradation of Glutathione in Nicotiana tabacum  
 Abstract    During degradation o f glutathione in tobacco suspension cultures substancial amounts o f 5-oxo-proline are formed in vivo as well as in crude cell homogenates in vitro. The existance o f a 5-oxo-prolinase that catalyzes the conversion of 5-oxo-proline to glutamic acid was demonstrated in tobacco cells, grown with glutathione as sole sulfur source. 
  Reference    Z. Naturforsch. 35c, 708—711 (1980); received June 18 1980 
  Published    1980 
  Keywords    5-Oxo-prolinase, Glutathione, Tobacco, Tissue Culture 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0708.pdf 
 Identifier    ZNC-1980-35c-0708 
 Volume    35 
3Author    E. Bengsch12, F. K. Orte2, J. Polster3, M. Schwenk3, V. ZinkernagelRequires cookie*
 Title    Reduction in Symptom Expression of Belladonna Mottle Virus Infection on Tobacco Plants by Boron Supply and the Antagonistic Action of Silicon  
 Abstract    Boron and silicon have a dram atic influence on the expression of symptoms caused by plant virus infections. For tobacco plants, boron decreases and silicon enhances symptom expression after belladonna m ottle virus infection. Infected leaf tissues are highly enriched on both trace elem ents. Boron supply stim ulates the silicon accumulation. In the applied concentrations the trace elem ents have no phytotoxic effects on the host. Except endogeneous ß-interferones, this could be the first example of antiviral action without any cytotoxicity. 
  Reference    Z. Naturforsch. 44c, 777—780 (1989); received February 13/April 18 1989 
  Published    1989 
  Keywords    Plant Virus Infection, B oron, Silicon, Tobacco, Belladonna M ottle Virus 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0777.pdf 
 Identifier    ZNC-1989-44c-0777 
 Volume    44 
4Author    K. Burda3-, K. Strzałkab, G. H. Schmid3Requires cookie*
 Title    Europium-and Dysprosium-Ions as Probes for the Study of Calcium Binding Sites in Photosystem II  
 Abstract    Trivalent lanthanide cations are suitable probes for C a2+-binding sites in photosystem II (PS II). PS II membranes prepared from Nicoticinci tabacum, intact and depleted of the extrinsic polypeptides were exposed to lanthanide ions (D y3+ and E u 3+). Small concen­ trations of dysprosium and europium ions enhance oxygen evolution under short saturating flashes. Higher concentrations of the rare earth cations cause the release of the three extrinsic peptides (17, 23 and 33 kDa) and reduce 0 2 yield. The reactivation of the PS II membranes, thus depleted of the 33 kDa subunit, by Ca2+ ions is not possible. Comparing E u 3+ with Dy3+ in this effect shows that E u 3+ is m ore effective than Dy3+, because a lower E u 3+-concentration in comparison to that of Dy3+ inactivates 0 2-evolution. The differences between europium and dysprosium can be explained by their different ionic radius. Our results suggest the existence of two Ca-binding regions: one with a low affinity for calcium would be located on the contact surface of the 23 and 33 kDa proteins and the second one with a high affinity, should be located close to the Mn-cluster and to tyrosine-161 (Z). The more tightly-bound calcium would be responsible for the activity of the PS II system. 
  Reference    Z. Naturforsch. 50c, 220 (1995); received O ctober 6/O ctob er 31 1994 
  Published    1995 
  Keywords    Calcium-Binding Sites, Photosystem II, Tobacco, Oxygen Evolution S-States 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0220.pdf 
 Identifier    ZNC-1995-50c-0220 
 Volume    50 
5Author    Vassya St, NedyalkaV. Bankova3, Dim Handjieva3, L. Itar, ZarkaY. Djilianovb, RositsaI. Vassilevab, AtanasI. Bachvarovab, Atanasov, KamenL. Stefanov3, SimeonS. Popov3Requires cookie*
 Title    Polyphenol and Alkaloid Changes in Glyphosate-Treated Tobacco Regenerants Selected for Herbicide Tolerance  
 Abstract    In vitro a one-step selection for glyphosate tolerance of two Bulgarian tobacco cultivars Zlatna Arda and Nevrokop A 24 was carried out. After treatment with glyphosate of the control plants and their regenerants polyphenol and alkaloid changes were determined. A decrease in the polyphenol concentrations and an in­ crease in the alkaloid concentrations were registered only for Zlatna Arda. 
  Reference    Z. Naturforsch. 50c, 313 (1995); received July 1/Novem ber 29 1994 
  Published    1995 
  Keywords    Glyphosate, Tobacco, in vitro Selection, Chlorogenic Acid, Rutin, Alkaloids 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0313_n.pdf 
 Identifier    ZNC-1995-50c-0313_n 
 Volume    50 
6Author    P. He, K. P. Bader, A. Radunz, G. H. SchmidRequires cookie*
 Title    Consequences of High C 0 2-Concentrations in Air on Growth and Gas-Exchange Rates in Tobacco Mutants  
 Abstract    Wild type tobacco N. tabacum var. John William's Broadleaf and the tobacco aurea mutant Su/su were permanently grown under 700 ppm C 0 2 in air. In comparison to plants grown under 350 ppm C 0 2 in air but under otherwise identical conditions growth was substantially enhanced. Gas exchange measurem ents carried out by mass spectrometry show that the rate of photosynthesis in the wild type and in the mutant is increased by more than 100%. The photorespiratory rate in the wild type measured as 180 2-uptake in the light in the "700 ppm C 0 2-plants" is not reduced to the extent expected or deduced from experiments in which the 350 ppm system responds under in vitro conditions to 700 ppm C 0 2. An analysis of the induction kinetics of room tem perature fluorescence kinetics of the adapted (700 ppm C 0 2) system and the control system (350 ppm C 0 2) under various C 0 2-partial pressures shows that perm anent growth under the elevated C 0 2-partial pressure leads to a structural modifi­ cation of the photosynthetic apparatus. 
  Reference    Z. Naturforsch. 50c, 781—788 (1995); received July 3/July 30 1995 
  Published    1995 
  Keywords    High C 0 2-Pressure, Mass Spectrometry, Tobacco, Photosynthesis, Photorespiration 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0781.pdf 
 Identifier    ZNC-1995-50c-0781 
 Volume    50 
7Author    K. Burda, G. H. SchmidRequires cookie*
 Title    On the Determination of the 5-State Distribution in the Kok Model  
 Abstract    We use the Markow chain theory to analyze the oscillation pattern of oxygen evolution during water oxidation in photosystem II under short saturating light flashes. We propose a method based on the standard least square deviation (test x 2) to determine the number of 5-states in the Kok model. As pointed out by Burda et al. (1995) this information is amongst others important for the interpretation of the role of calcium for oxygen evolution. A specific mathematical representation for a situation when the S4 state is longer living than generally assumed is introduced which requires an explicit extension of the Kok model to five states. The higher stability is modelled by introducing additional decay channels, e.g. a nonvanishing probability for the transition of S3 to the 5() state and a further transition probability for the transition from S3 to 5 4. Our analysis is extended to the case of damped oscillations of oxygen evolution caused, for example, by the lack of electron acceptor or the short life time of photosystem II particles. 
  Reference    Z. Naturforsch. 51c, 329—341 (1996); received O ctober 23/D ecem ber 5 1995 
  Published    1996 
  Keywords    0 2-Evolution, o-and ^-Analysis, Transition Probabilities, Cyanobacteria, Tobacco, Chlorella 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0329.pdf 
 Identifier    ZNC-1996-51c-0329 
 Volume    51