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'Tobacco Mosaic Viruses' in keywords
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1980 (2)
1Author    AlanC. Cassells, FrancesM. CockerRequires cookie*
 Title    TMV Inoculation of Tobacco Protoplasts in the Presence of Protoplast Fusion Agents  
 Abstract    Two methods used to induce protoplast fusion, involving polyethylene glycol (PEG) and high calcium/high pH, respectively, have been investigated in procedures for the inoculation of isolated tobacco protoplasts with TMV. It has been shown that inoculation in the presence of fusion agents involves two stages. Firstly, virus precipitation on to the protoplast surface and secondly, entrapment of virus particles between protoplasts in zones (putatively) o f transient protoplast fusion. 
  Reference    Z. Naturforsch. 35c, 1057—1061 (1980); received June 24/August 22 1980 
  Published    1980 
  Keywords    Polyethylene Glycol, Protoplasts, Tobacco Mosaic Virus 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-1057.pdf 
 Identifier    ZNC-1980-35c-1057 
 Volume    35 
2Author    U. Kreibig, C. W. EtterRequires cookie*
 Title    Light Diffraction of in vitro Crystals of Six Tobacco Mosaic Viruses  
 Abstract    Iridescent gels o f the common, tomato mosaic, para-tobacco mosaic, ribgrass mosaic, sunnhemp mosaic, and cucumber 4 tobacco mosaic virus strains were prepared using the purification method of Boedtker and Simmons (J. Amer. Chem. Soc. 1958). Macrocrystals which were stable for many months could be grown from iridescent gels of all viruses when stored at 0 -1 0 °C. The gels exhibited various structural phases (nematic and smectic) differing in density. The structure of the microcrystalline gels and of macrocrystals was investigated by means of optical diffraction. Distinct Bragg reflections were observed from which a mean periodicity of 340 nm was derived for all virus strains. The results indicate a crystalline multilayer structure as first proposed by Oster (J. Gen. Physiol. 33, 445, [1950]). The angular positions and widths of the Bragg reflections depended on the concentration of virus and the ionic strength. The periodicity decreased from 365 nm at 30 mg/ml virus to about 315 nm at 109 mg/ml virus, i.e. the periodicity approached the 300 nm length o f the virion. Model calculations taking into account Debye-Hückel repulsive and van der Waals attractive forces gave a qualitative explanation for the crystalline structure and its observed concentration dependence. The in vitro crystals resemble immature crystal forms observed in the living cell. 
  Reference    Z. Naturforsch. 35c, 750—762 (1980); received March 7 1980 
  Published    1980 
  Keywords    Tobacco Mosaic Viruses, Crystallization, Phase Transitions, Light Diffraction, Crystal Structure, Model Calculations 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0750.pdf 
 Identifier    ZNC-1980-35c-0750 
 Volume    35