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'Suspension Cultures' in keywords Facet   Publication Year 1983  [X]
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1Author    Bernd Knoop, R. Olf BeiderbeckRequires cookie*
 Title    Adsorbenskultur — ein W eg zur Steigerung der Sekundärstoffproduktion in pflanzlichen Suspensionskulturen Adsorbent Culture -M ethod for the Enhanced Production of Secondary Substances in Plant Suspension Cultures  
 Abstract    A crown-gall suspension o f M atricaria cham omilla L. was cultivated in the presence o f an adsorbent (activated charcoal, AC). The culture produces besides other secondary products coniferyl aldehyde which is excreted by the cells and distributed between cells, m edium , and the AC according to equilibrium distributions with more than 95% o f the substance adsorbed to the AC. This deprivation o f coniferyl aldehyde from the m edium enables the cells to increase the production o f this substance up to 60-fold. The system M atricaria/coniferyl aldehyde serves as a model for the improvement o f the production o f secondary products by means o f plant cell suspensions. 
  Reference    Z. Naturforsch. 38c, 484 (1983); received February 10 1983 
  Published    1983 
  Keywords    Matricaria, Suspension Culture, Coniferyl Aldehyde, Accumulation, Adsorbent 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0484_n.pdf 
 Identifier    ZNC-1983-38c-0484_n 
 Volume    38 
2Author    Jochen Berlin, Edgar Forche, Victor Wray, Joachim Hammer, Wolfgang HöselRequires cookie*
 Title    Formation of Benzophenanthridine Alkaloids by Suspension Cultures o f Eschscholtzia californica  
 Abstract    Suspension cultures of Eschscholtzia californica accum ulate the dihydro forms o f the benzo­ phenanthridine alkaloids sanguinarine, chelirubine, macarpine and chelerythrine, all o f which are known to be constituents o f the Eschscholtzia plant. U nder most experimental conditions dihydrochelirubine was found to be the main constituent of the cultured cells. The specific yields of alkaloids varied from zero to 1.7% on a dry weight basis depending on the m edia conditions. The highest specific yield was 1.5 m g/g dry weight or 13 mg/1 with the growth m edium B5. After transfer of the cells into the induction medium IM2 the alkaloid accum ulation increased to 17 mg/g dry weight and 146 mg/1. The induction m edium contained increased levels o f sucrose, decreased levels of phosphate and was devoid of phytohormones. The effect o f the various m edia conditions on the biosynthesis of phenolics was quite different to those found for the alkaloids. 
  Reference    Z. Naturforsch. 38c, 346 (1983); received February 7 1983 
  Published    1983 
  Keywords    Eschscholtzia californica, Suspension Cultures, Benzophenanthridine Alkaloids, Phenolics, Media Effects 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0346.pdf 
 Identifier    ZNC-1983-38c-0346 
 Volume    38 
3Author    W. N. Oé, H. U. SeitzRequires cookie*
 Title    Studies on the Regulatory Role of fra/is-Cinnamic Acid on the Activity of the Phenylalanine Ammonia-Lyase (PAL) in Suspension Cultures of Daucus carota L  
 Abstract    In vivo and in vitro experiments were performed in order to study the regulatory role of trans-cinnamic acid and its hydroxylated derivatives (p-coumaric acid, caffeic acid) on the deamina­ tion of phenylalanine catalyzed by PAL (EC 7>arts-cinnamic acid inhibits growth and reduces the content of soluble proteins of anthocyanin-containing carrot cells grown in suspen­ sion. There is strong evidence from the polysomal patterns and from the effect of ?ra«5-cinnamic acid on protein synthesis in vitro that protein synthesis is inhibited. The kinetic data of PAL clearly demonstrate that /ra«s-cinnamic acid inhibits the enzyme by a noncompetitive mecha­ nism. On the contrary, L-a-aminooxy-/?-phenylpropionic acid (l-AOPP), a competitive inhibitor of PAL, does not affect protein metabolism. 
  Reference    Z. Naturforsch. 38c, 408 (1983); received January 24 1983 
  Published    1983 
  Keywords    Phenylalanine Ammonia-Lyase, Protein Metabolism, Regulation, /ra/js-Cinnamic Acid, Daucus carota, Suspension Culture 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0408.pdf 
 Identifier    ZNC-1983-38c-0408 
 Volume    38