| | 1 | Author
| Divya Mishra, Ahlert Schmidt | Requires cookie* | | | Title
| Regulation and Partly Purification of the ATP-Sulfurylase from the Cyanobacterium Synechococcus 6301  | | | | Abstract
| ATP-sulfurylase from the cyanobacterium Synechococcus 6301 was regulated in vivo during growth in batch culture. The activity was highest at the third day after inoculation, declining afterwards to a level found in resting cells. During growth with air supplemented with 2% CO, this activity increased 3-fold compared to controls grown with normal air as C 0 2 source. Addition o f either nitrite or urea enhanced ATP-sulfurylase activity about 2-fold, whereas cys teine and especially methionine decreased ATP-sulfurylase activity to 5% o f controls without treatment. The ATP-sulfurylase was purified by conventional techniques using D EAE-cellulose chro matography and further separation on blue sepharose achieving a 250-fold increase in the spe cific activity. An apparent o f 5 for APS and o f 40 |iM for pyrophosphate was deter mined with the purified enzyme fraction. | | | |
Reference
| Z. Naturforsch. 47c, 95 (1992); received September 26 1991 | | | |
Published
| 1992 | | | |
Keywords
| ATP-Sulfurylase, Cyanobacterium, Synechococcus, Sulfate A ctivation, Regulation | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0095.pdf | | | | Identifier
| ZNC-1992-47c-0095 | | | | Volume
| 47 | |
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