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1991 (1)
1Author    Susanne Bickel-Sandkötter, K. Ordula Esser, M. Artina, H. OrbachRequires cookie*
 Title    On the Accessibility of Essential Tyrosines in Isolated and Activated Chloroplast H +-ATPase  
 Abstract    The addition o f 7-chloro-4-nitrobenzofurazan to isolated and activated CF, creates a com ­ pletely changed binding stoichiometry and subunit-distribution o f bound modifier in contrast to the binding pattern in not-activated CF,. The activation o f CF, by dithiothreitol and heat results in the accessibility o f three additional tyrosines in ß-subunits and one additional tyro­ sine in a-subunits. Binding o f pyridoxalphosphate to lysine in the active state suppresses the accessibility o f the additional tyrosines, suggesting that PLP inactivates the ATPase by induc­ ing a conformational change. Furthermore, two N BD -m olecules are bound to y-subunits when CF, is activated. These two molecules may be bound to sulfhydryl groups o f cysteines which become accessible to N B D after activation. 
  Reference    Z. Naturforsch. 46c, 71—78 (1991); received September 7 1990 
  Published    1991 
  Keywords    ATP-Hydrolysis, ATPase, Chloroplast, Covalent M odification, Subunit Labeling 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0071.pdf 
 Identifier    ZNC-1991-46c-0071 
 Volume    46