Go toArchive
Browse byFacets
Bookbag ( 0 )
'Substrate Specificity' in keywords Facet   section ZfN Section C  [X]
Facet   Publication Year 1990  [X]
Results  1 Item
Sorted by   
Publication Year
1Author    PaulL D Urham, JonathanE. PoultonRequires cookie*
 Title    Enzymic Properties of Purified Myrosinase from Lepidium sativum Seedlings  
 Abstract    To establish the substrate specificity o f the thioglucoside glucohydrolase myrosinase (EC, this enzyme was purified to homogeneity from light-grown cress (Lepidium sativum L.) seedlings by Sephadex gel filtration. Red Dye and anion exchange (FPLC M ono Q) chro­ matography, and preparative isoelectric focusing. Hydrolytic activity was shown toward only 4 o f the 29 synthetic and natural O-and S-glycosides tested. Highest activity was displayed with the endogenous glucosinolates benzylglucosinolate (ATm, 295 |iM) and sinigrin (K ml 300 |iM) at an optimum pH o f 5.5 in sodium citrate buffer. The synthetic glycosides PNPG (K m, 2.0 m M) and ONPG were poorer substrates at an optimum pH o f 6.5 in potassium phos­ phate buffer. The enzyme was inactive with all other nitrophenyl glycosides tested including PNP-a-D-glucoside and PNP-thio-ß-D-glucoside, suggesting a requirement for O-ß-D-glucose as the glycone moiety within these substrates. PNPG hydrolysis was stimulated 2.6-fold by ascorbate (1 m M). The enzyme exhibited no metal ion requirement and was strongly inhibited by lead nitrate, mercury chloride, and ferric chloride at 1 m M concentration. The metal chela­ tors D IE C A , ED TA , o-phenanthroline, and 2,2'-dipyridyl were not inhibitory, but the thiol reagents PCM S, PCMB, and N-ethylmaleimide (at 1 m M) caused 5 0 -8 0 % inhibition o f enzyme activity. 
  Reference    Z. Naturforsch. 45c, 173—178 (1990); received September 9 1989 
  Published    1990 
  Keywords    Lepidium sativum, Thioglucoside Glucohydrolase, M yrosinase, Substrate Specificity, Gluco-sinolate Degradation 
  Similar Items    Find
 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0173.pdf 
 Identifier    ZNC-1990-45c-0173 
 Volume    45