ZfN: Search Results

1	Author	KenjiM. Atsui, Hiroyuki Shinta, H. Irom, Itsu Toyota, Tadahiko Kajiwara, AkikazuH. Atanaka	Requires cookie*
	Title	Comparison of the Substrate Specificities of Lipoxygenases Purified from Soybean Seed, Wheat Seed, and Cucumber Cotyledons
	Abstract	Lipoxygenases were highly purified from soybean seed, wheat seed and cucumber cotyle dons. Substrate specificities o f these lipoxygenases were studied by using an entire series o f (co6Z ,co9Z)-C 13~C24-dienoic acids as synthetic substrate analogues. Soybean lipoxygenase-1 and cucumber lipoxygenase showed broad specificities for these substrates while wheat lipoxygenase showed narrow specificities. Position o f dioxygenation to each substrate was an alyzed by high performance liquid chromatography. W ith soybean lipoxygenase-1 elongation o f the distance between the terminal carboxyl group and the site o f hydrogen removal in a substrate decreased the positional specificity o f dioxygenation, while, with cucumber lipoxy genase, shortening the distance decreased the specificity. It was suggested that cucumber lipoxygenase and soybean lipoxygenase-1 recognized the terminal carboxyl group o f a sub strate to arrange it only in one orientation at the reaction center. In case o f wheat lipoxygen ase, recognition o f the carboxyl group was thought to have crucial and essential role to secure the activity.
	Reference	Z. Naturforsch. 47c, 85—8 (1992); received M ay 8/A ugust 27 1991
	Published	1992
	Keywords	Lipoxygenase, Cucumber Cotyledons, Soybean Seed, W heat Seed, Substrate Specificity
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	TEI-XML for	default:Reihe_C/47/ZNC-1992-47c-0085.pdf
	Identifier	ZNC-1992-47c-0085
	Volume	47

2	Author	AkikazuH. Atanaka, T. Adahiko Kajiwara, Kenji Matsui, Hiromitsu Toyota	Requires cookie*
	Title	Substrate Specificity of Tea Leaf Hydroperoxide Lyase
	Abstract	Substrate specificity o f tea leaf fatty acid hydroperoxide lyase was systematically investigat ed using an entire series o f co6-(5)-hydroperoxy-C14-C24 dienoic and trienoic acids as sub strates. Unexpectedly, the hydroperoxides o f C22 but not natural substrates, i.e., those o f C 18, showed the highest reactivities for the lyase. The reactivities o f the hydroperoxides o f trienoic acids were always four to ten times higher than those o f the dienoic acids.
	Reference	Z. Naturforsch. 47c, 677—6 (1992); received June 24/July 24 1992
	Published	1992
	Keywords	Aldehyde, Fatty Acid Hydroperoxide, Hydroperoxide Lyase, Substrate Specificity, Tea Leaf
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	TEI-XML for	default:Reihe_C/47/ZNC-1992-47c-0677.pdf
	Identifier	ZNC-1992-47c-0677
	Volume	47