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'Substrate Specificity' in keywords Facet   Publication Year 1992  [X]
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1992[X]
1Author    KenjiM. Atsui, Hiroyuki Shinta, H. Irom, Itsu Toyota, Tadahiko Kajiwara, AkikazuH. AtanakaRequires cookie*
 Title    Comparison of the Substrate Specificities of Lipoxygenases Purified from Soybean Seed, Wheat Seed, and Cucumber Cotyledons  
 Abstract    Lipoxygenases were highly purified from soybean seed, wheat seed and cucumber cotyle­ dons. Substrate specificities o f these lipoxygenases were studied by using an entire series o f (co6Z ,co9Z)-C 13~C24-dienoic acids as synthetic substrate analogues. Soybean lipoxygenase-1 and cucumber lipoxygenase showed broad specificities for these substrates while wheat lipoxygenase showed narrow specificities. Position o f dioxygenation to each substrate was an­ alyzed by high performance liquid chromatography. W ith soybean lipoxygenase-1 elongation o f the distance between the terminal carboxyl group and the site o f hydrogen removal in a substrate decreased the positional specificity o f dioxygenation, while, with cucumber lipoxy­ genase, shortening the distance decreased the specificity. It was suggested that cucumber lipoxygenase and soybean lipoxygenase-1 recognized the terminal carboxyl group o f a sub­ strate to arrange it only in one orientation at the reaction center. In case o f wheat lipoxygen­ ase, recognition o f the carboxyl group was thought to have crucial and essential role to secure the activity. 
  Reference    Z. Naturforsch. 47c, 85—8 (1992); received M ay 8/A ugust 27 1991 
  Published    1992 
  Keywords    Lipoxygenase, Cucumber Cotyledons, Soybean Seed, W heat Seed, Substrate Specificity 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0085.pdf 
 Identifier    ZNC-1992-47c-0085 
 Volume    47 
2Author    AkikazuH. Atanaka, T. Adahiko Kajiwara, Kenji Matsui, Hiromitsu ToyotaRequires cookie*
 Title    Substrate Specificity of Tea Leaf Hydroperoxide Lyase  
 Abstract    Substrate specificity o f tea leaf fatty acid hydroperoxide lyase was systematically investigat­ ed using an entire series o f co6-(5)-hydroperoxy-C14-C24 dienoic and trienoic acids as sub­ strates. Unexpectedly, the hydroperoxides o f C22 but not natural substrates, i.e., those o f C 18, showed the highest reactivities for the lyase. The reactivities o f the hydroperoxides o f trienoic acids were always four to ten times higher than those o f the dienoic acids. 
  Reference    Z. Naturforsch. 47c, 677—6 (1992); received June 24/July 24 1992 
  Published    1992 
  Keywords    Aldehyde, Fatty Acid Hydroperoxide, Hydroperoxide Lyase, Substrate Specificity, Tea Leaf 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0677.pdf 
 Identifier    ZNC-1992-47c-0677 
 Volume    47