| | 1 | Author
| Akira Taketo, Yoriko Taketo | Requires cookie* | | | Title
| Effect of Streptococcal Extracellular Nuclease on the Carrier Activity of RNA for Streptolysin S  | | | | Abstract
| Upon digestion with a streptococcal extracellular nuclease, yeast RNA yielded acid-insoluble core having increased carrier activity for streptolysin S. The carrier activity was found in minor fractions of the core which were eluted from a DEAE-cellulose column at higher salt concentrations. Upon gel filtration through a Sephadex G-75 column, the effective component (Fr. I) was eluted earlier than bulk oligonucleotides (Fr. II). Nucleotide composition (in mol %) of Fr. I was AMP: 21.8; GMP: 55.1; CMP: 8.2; UMP: 14.9, whereas that o f Fr. II was AMP: 38.0; GMP: 33.1; CMP: 8.0; UMP: 20.9. Chromatographic patterns of SLS complex induced by Fr. I were similar to those of the toxin formed in the presence o f active fraction prepared from RNase I core. Hemolytic activity of the latter complex was, like the former, unaffected by streptococcal nuclease treatment. The carrier activity o f DNA digested with the nuclease was also investigated. | | | |
Reference
| Z. Naturforsch. 38c, 107 (1983); received October 1 1982 | | | |
Published
| 1983 | | | |
Keywords
| Streptolysin S, RNA Effect, Extracellular Nuclease, Streptococcus hemolyticus | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0107.pdf | | | | Identifier
| ZNC-1983-38c-0107 | | | | Volume
| 38 | |
| 2 | Author
| Akira Taketo, Yoriko Taketo | Requires cookie* | | | Title
| Effects of Membrane-Acting Drugs and Aerobiosis on Production of Streptolysin S and Nuclease in Hemolytic Streptococci | | | | Abstract
| Yield of streptolysin S (SLS) in streptococcal culture was considerably reduced by procaine, dibucaine, atropine or chlorpromazine at concentrations which scarecely affected production of an extracellular nuclease as well as the bacterial growth. Cerulenin was also inhibitory to SLS formation, but its effect was more pronounced on the nuclease production. By aerobiosis, amount of SLS produced into culture supernatant was increased significantly, whereas yield of the nuclease was rather unaffected. | | | |
Reference
| Z. Naturforsch. 39c, 1128—1131 (1984); received August 14 1984 | | | |
Published
| 1984 | | | |
Keywords
| Streptolysin S, Nuclease, Membrane, Aerobiosis, Streptococci | | | |
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| | | | | TEI-XML for
| default:Reihe_C/39/ZNC-1984-39c-1128.pdf | | | | Identifier
| ZNC-1984-39c-1128 | | | | Volume
| 39 | |
| 3 | Author
| Akira Taketo, Yoriko Taketo | Requires cookie* | | | Title
| Enhanced Production of Cell-Bound and Extracellular Streptolysin S by Hemolytic Streptococci Pretreated with Proteases | | | | Abstract
| The amount o f streptolysin S produced by resting streptococci was considerably increased after incubation o f the washed bacteria with trypsin or pronase. Production o f both cell-bound and free forms o f the toxin was enhanced by the protease treatment. By addition o f trypsin, streptolysin S yield was considerably increased in growing culture as well. Treatment with lysozyme was ineffective, and the toxin production was only slightly prom oted by preincubation with hyaluronidase or Chymotrypsin. In contrast, pretreatment with chymotrypsin caused in creased production o f an extracellular nuclease, whereas the yield o f this enzyme was reduced after incubation o f the cocci with pronase. Evidence was obtained indicating de novo synthesis o f the exotoxin in the protease-treated bacteria. | | | |
Reference
| Z. Naturforsch. 40c, 166 (1985); received N ovem ber 22 1984 | | | |
Published
| 1985 | | | |
Keywords
| Streptolysin S, Cell-Bound Hemolysin, Protease, Streptococci | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0166.pdf | | | | Identifier
| ZNC-1985-40c-0166 | | | | Volume
| 40 | |
| 4 | Author
| Yoriko Taketo, Akira Taketo | Requires cookie* | | | Title
| Effects of Polynucleotides on Production and Activity of Streptolysin S | | | | Abstract
| Activity o f various natural and synthetic polynucleotides as a carrier for streptolysin S was tested in a resting cell system. As the carrier, intact m olecules o f M S2 R N A , E. coli tR N A or rat liver R N A were almost inactive, whereas R N ase I core o f these R N A s, especially the core frac tions eluted from DEA E-cellulose colum n at higher NaC l concentrations, effectively induced pro duction o f the extracellular hem olysin. The carrier activity o f yeast R N A was significantly en hanced by sim ultaneous addition o f R N ase I, to the streptococcal suspension. Preincubation o f yeast R N A with growing streptococci or a protein fraction from the bacterial culture supernatant increased its carrier activity. Evidences were obtained suggesting involvem ent o f streptococcal nuclease in the enhancement o f the carrier effect. Production o f the streptococcal hem olysin was markedly prom oted by polyguanylic acid but neither by polyadenylic acid, polycytidylic acid nor by polyuridylic acid. Like trypan blue, polyguanylic acid exerted potent inhibitory effect on hem olytic activity o f streptolysin S complex. Similar but less marked effect was observed with certain R N ase I core fractions o f rat liver R N A. Som e data concerning effect o f polynucleotides on stability o f the hem olysin were also presented. | | | |
Reference
| Z. Naturforsch. 35c, 578 (1980); received March 10 1980 | | | |
Published
| 1980 | | | |
Keywords
| Streptolysin S, R N A, Polyguanylic Acid, Streptococcus pyogenes | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0578.pdf | | | | Identifier
| ZNC-1980-35c-0578 | | | | Volume
| 35 | |
| 5 | Author
| Akira Taketo, Yoriko Taketo | Requires cookie* | | | Title
| Decreased Production of RNA-Streptolysin S in Streptococci Devoid of Extracellular RNase Activity | | | | Abstract
| In cultures of certain streptococcal strains, R N A added as a carrier for streptolysin S (SLS) was hardly degraded, owing to deficiency of extracellular RNase activity. Production of RNA-SLS into culture supernatant was markedly reduced in the RNase-deficient streptococci. Even in these RNase-less strains, guanylic-acid rich oligonucleotides, polyG or trypan blue effectively induced SLS production, as in RNase-positive cells. These results demonstrate involvement of the streptococcal nuclease in manifestation of SLS-inducing effect of exogenous RNA. Additional data indicated that cellular growth was promoted by supplementation of RNA, in the nuclease-producing streptococci. | | | |
Reference
| Z. Naturforsch. 38c, 635—639 (1983); received December 6 1982/March 1 1983 | | | |
Published
| 1983 | | | |
Keywords
| Streptolysin S, Extracellular Nuclease, RN A Effect, Streptococcus hem olyticus | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0635.pdf | | | | Identifier
| ZNC-1983-38c-0635 | | | | Volume
| 38 | |
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