| | 1 | Author
| Alexandru Popescu, Stefan Antohi, Stefan Trasculescu, Adriana Aurescu, NicolaeM. Anolescu, Victor Ciocnitu | Requires cookie* | | | Title
| Changes Induced by Pre-Fixation in Polycation Bacteriolysis and Surface Alterations Matching the Model of Wall Picnosis in Cell Lysis  | | | | Abstract
| Pretreatments of B. subtilis and S. aureus cells with lower concentrations of fixative agents, led to modifications in bacteriolytic effect exerted by polyarginine and protamine: Glutaraldehyde blocked polycation bacteriolysis while formaldehyde and osmium tetroxide (0 s0 4) having no influence on polyarginine action, increased constantly the cell sensitivity to protamine in lower doses otherwise nonlytic; the sensitizing action also resulted in the extension of protamine bacteriolytic pattern including several staphylococcal strains; higher bacteriolytic doses of protamine were contrastively unable to lyse OsO? prefixed cells and gave an inconstant lytic value with formaldehyde treated bacteria. With higher concentrations, 0 s 0 4 preserved intactly its sensitizing action while formaldehyde displayed a decrease in its ability to sensitize B. subtilis cells to the lytic effect of protamine. Scaning electron microscopy of polycation treated cells showed prelytic lesions as surface granulations, shape and size modifications and cell splits. The interpretation of the results in terms of intra-and intermolecular adducts accompanied by con formational changes in surface macromolecules is discussed. It is concluded that the results match the model of polycation bacteriolysis by wall multizonal picnosis leading to surface splits and thereby triggering cell-lysis. | | | |
Reference
| Z. Naturforsch. 35c, 805—810 (1980); received July 9 1980 | | | |
Published
| 1980 | | | |
Keywords
| Poly-L-arginine, Protamine, Bacillus subtilis, Staphylococcus aureus, Glutaraldehyde | | | |
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| | | | | TEI-XML for
| default:Reihe_C/35/ZNC-1980-35c-0805.pdf | | | | Identifier
| ZNC-1980-35c-0805 | | | | Volume
| 35 | |
| 2 | Author
| Heinz Egge, Uwe Murawski, Wirapong Chatranon, Fritz Zilliken | Requires cookie* | | | Title
| eine gaschromatographisch-massenspektrometrische Analyse The Branched Chain Fatty Acids of Staphylococcus aureus, a Gas Chromato- graphic-Mass Spectrometric Analysis | | | | Abstract
| The fatty acid methylesters prepared by transesterification of the total lipid extract of Staphylo-coccus aureus Wood 46 were analysed by gas chromatography on thin film capillaries coated with polyphenylether (OS 138), siliconerubber (SE 30) and S.C.O.T. columns coated with Diethylene-glycoladipatepolyester (DEGA) in combination with mass spectrometry. Among the branched chain fatty acids ranging from Clt — C21, the iso-and anteiso-C15 acids are predominant. In the group of the C^ and C17 acids branching in position 9 and 10 was also found. No unsaturated branched chain fatty acids could be detected. Retention data and mass spectra of the branched chain fatty acids are presented. Die Neutrallipide, Phospholipide und Glycero-glykolipide der Staphylococcen enthalten einen gro-ßen Anteil verzweigter Fettsäuren, bei denen wo-und a/itewo-Fettsäuren vorherrschen 2 . Die quan-titative Verteilung der Lipide und der Fettsäuren ist | | | |
Reference
| (Z. Naturforsch. 26b, 893—901 [1971]; eingegangen am 6. März 1971 revidiert am 18. Mai 1971) | | | |
Published
| 1971 | | | |
Keywords
| Staphylococcus aureus, Branched chain fatty acids, Gas chromatography, Capillary columns, Mass spectrometry | | | |
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| default:Reihe_B/26/ZNB-1971-26b-0893.pdf | | | | Identifier
| ZNB-1971-26b-0893 | | | | Volume
| 26 | |
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