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'Standard Curves for Characterization o f Chlorophyll Proteins' in keywords Facet   Publication Year 1980  [X]
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1Author    Aloysius Wild, Barbara UrschelRequires cookie*
 Title    Chlorophyll-Protein Complexes of Chlorella fusca  
 Abstract    Chlorophyll-protein complexes from thylakoids of the normal type and two mutants o f Chlorella fusca were separated using sodium dodecyl sulfate acrylamide gel electrophoresis (PAGE). The properties o f the chlorophyll-protein complexes o f the three strains of Chlorella were compared. Standard curves were set up for the characterization of the chlorophyll-proteins. In every electrophoretic separation of chlorophyll-protein complexes, a certain am ount o f pigm ent is separated from the protein. We tried to keep that am ount as low as possible by mild solubiliza­ tion and by working in low tem perature. U nder these conditions, we obtained several new chlorophyll-proteins in addition to the P-700-chlorophyll a-protein (CP I) and the light-harvesting chlorophyll a/b-protein (CP II). Thus, a small band (CP I a) was located between the top of the gel and the CP I after elec­ trophoresis. A lthough it shows spectral qualities sim ilar to CP I, it possesses a much lower chloro­ phyll a/chlorophyll b ratio. It may be an aggregate of photosystem I and light-harvesting chloro­ phyll. We found and analyzed three other chlorophyll-proteins with electrophoretic m obilities inter­ mediate between that o f the P-700-chlorophyll a-protein and that of the light-harvesting chloro­ phyll a/b-protein complex. Two o f these chlorophyll-proteins, the LHCP1 and the L H C P2, have a low chlorophyll a/chlorophyll b ratio and spectral properties similar to that o f the light-harvesting chlorophyll a/b-protein (LH CP3). They obviously represent dimers or oligomers o f the latter com ­ plex. A third, new complex (CPa) m igrated between LHCP3 and its dimer. W ith the chlorophyll b deficient m utant G 36 of Chlorella fusca, this complex could be obtained in high purity and great enrichment (15% of total chlorophyll). It could be proved that this complex only contains chloro­ phyll a. Its red absorption m axim um is at 671 nm. Some indirect evidences suggest that it may be a good candidate for the PS II reaction center complex. 
  Reference    Z. Naturforsch. 35c, 627—6 (1980); received March 19 1980 
  Published    1980 
  Keywords    Chlorella, C hlorophyll-Protein Complexes, Polyacrylam ide-Gel Electrophoresis, Standard Curves for Characterization o f Chlorophyll Proteins 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0627.pdf 
 Identifier    ZNC-1980-35c-0627 
 Volume    35