| | 1 | Author
| K. L. Friedm, R. Egel | Requires cookie* | | | Title
| Protein Patterns during Sporulation in Fission Yeast  | | | | Abstract
| Various strains (wild-type and m utants) of Schizosaccharom yces pom be were subjected to sporulation conditions, and analysed with regard to changes in their protein patterns. Trisbuffer-soluble proteins (TS) and DNA-binding proteins (DB) were denatured and separated by poly acrylam ide electrophoresis. A fter endogenous nitrogen depletion (the apparent trigger of conjuga tion and sporulation) the following alterations were observed: larger TS-proteins were proteolytical-ly degraded; the pattern of DB-proteins underwent several selective changes (reduction, enforce ment, or new appearance of individual bands) ; an endonucleolytic DNase activity appeared. Most of these changes were expressed before conjugation, and did not appear in sterile strains. They could, however, be provoked by an abrupt shift to nitrogen-free medium, even in sterile strains. Inhibition studies with cycloheximide revealed at least two phases of protein synthesis specifically needed for sporulation: a very sensitive phase before conjugation, and a more resistant phase lasting until 0.5 h before spore form ation. Medium exchange between heterothallic cultures (h+ and h~) effected certain sporulation-specific alterations of the DB-protein pattern, indicative of diffusible mating-type factors in the culture fluid. | | | |
Reference
| Z. Naturforsch. 33c, 84 (1978); received December 14 1977 | | | |
Published
| 1978 | | | |
Keywords
| Schizosaccharom yces pom be, Conjugation, Sporulation, Endonuclease, Gel Electrophoresis | | | |
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| default:Reihe_C/33/ZNC-1978-33c-0084.pdf | | | | Identifier
| ZNC-1978-33c-0084 | | | | Volume
| 33 | |
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