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1Author    G. K. Ulandaivelu An, D.0 H AllRequires cookie*
 Title    Ultrastructural Changes in in vitro Ageing Spinach Chloroplasts  
 Abstract    Ultrastructural changes in in vitro ageing spinach chloroplasts have been studied in detail. Prolonged storage caused swelling of the chloroplasts due to the increase in the thickness and spacing of the thylakoid membranes. The increase in the thickness of the membrane is partly ac­ companied by the release of lipids. Addition of crystalline bovine serum albumin was found to stabilize the membrane structures. Storage of the chloroplasts at 77 °K even though it resulted in complete breakage of the whole chloroplasts, maintained the thylakoid structures in a highly intact form. 
  Reference    (Z. Naturforsch. 31c, 82 [1976]; received August 8 1975) 
  Published    1976 
  Keywords    Ultrastructure, Thylakoid Membrane, Ageing, Storage Conditions, Spinach 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0082.pdf 
 Identifier    ZNC-1976-31c-0082 
 Volume    31 
2Author    R. Thauer, H. Schirrmacher, W. Schymanski, P. SchönheitRequires cookie*
 Title    A Rapid Procedure for the Purification of Ferredoxin from Spinach Using Polyethyleneimine  
 Abstract    A rapid procedure for the purification of ferredoxin from spinach is described, which is based on the finding that ferredoxins can be precipitated with polyethyleneimines from acetone containing solutions. The ferredoxin was obtained in high yields (25 — 35 mg/kg spinach) and high purity (A 420/ A 27S= O . 4 7) within less than 8 hours. 
  Reference    Z. Naturforsch. 33c, 495—497 (1978); received M ay 17 1978 
  Published    1978 
  Keywords    Ferredoxin, Iron-Sulfur Proteins, Spinach, Photosynthesis, Polyethyleneimine 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0495.pdf 
 Identifier    ZNC-1978-33c-0495 
 Volume    33 
3Author    H. Gimmler, B. Heilmann, B. Demmig, W. HartungRequires cookie*
 Title    The Permeability Coefficients of the Plasmalemma and the Chloroplast Envelope of Spinach Mesophyll Cells for Phytohormones  
 Abstract    The uptake of auxin (LAA), abscisic acid (ABA) the synthetic cytokinin benzylaminopurine (BA), gibberellic acid (GA3) and mevalonic acid (lactone) (MVA) into intact chloroplasts and protoplasts from spinach mesophyll cells was measured and the permeability coefficients Ps of the chloroplast envelope and the plasmalemma were calculated. With all solutes tested uptake and P s values were considerably higher in the chloroplast system than in the protoplast system. At an external pH of 7.0, rates of uptake exhibited the order BA > LAA > MVA > ABA > GA3 in both systems. However, the P s values (corrected for the undissociated species of the solutes) exhibited the order LAA > GA3 > (ABA or BA) > MVA. This corrected sequence indicates the theoretical capacity of penetration under the assumption that preferentially the protonated species of phytohormones are capable of readily penetrating membranes. P s values for phytohormones appeared largely to be determined by the distribution coefficient K d and to a lesser extent by the molecular weight (Mr). In the Collander-plot the relation between the logarithm of Ps values for phytohormones and some other solutes such as acetate, glycerol, glucose, sorbitol and sucrose and the logarithm of K J M T 1.5 approached linearity. 
  Reference    Z. Naturforsch. 36c, 672—678 (1981); received March 9 1981 
  Published    1981 
  Keywords    Chloroplast Envelope, Permeability Coefficients, Phytohormones, Plasmalemma, Spinach 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0672.pdf 
 Identifier    ZNC-1981-36c-0672 
 Volume    36 
4Author    Kirsten Heim, G. Eorg, Kreim Er, M. Ichael, M. Elkonian, Erwin LatzkoRequires cookie*
 Title    Light-Induced Ca2+ Influx into Spinach Protoplasts  
 Abstract    Protoplasts from spinach leaves exhibit a light-induced Ca2+ influx. The half maximum rate of C a2+ influx is achieved at ~ 5 W m"2. The action spectrum o f this influx is similar to that of photosynthesis. Furtherm ore, light-induced Ca2+ influx is abolished by D C M U (2* 0.5 ^ im) and is sensitive to the uncoupler FCCP. Vanadate up to 3 enhances light-induced Ca2+ influx. These data indicate that photosynthetic electron transport is involved in light-induced Ca2+ influx into spinach protoplasts. 
  Reference    Z. Naturforsch. 42c, 283 (1987); received O ctober 24 1986 
  Published    1987 
  Keywords    Calcium-Influx, Protoplasts, Spinach, Light-Induced 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0283.pdf 
 Identifier    ZNC-1987-42c-0283 
 Volume    42 
5Author    E. Bengsch, J. Polster, M. SchwenkRequires cookie*
 Title    Effects of Simultaneous Supply of Silicon and Boron on Plant Growth and on Herbicide Toxicity  
 Abstract    An optimal supply of biodisposable silicon and boron shows a synergistic stimulation effect on plant growth. U nder these optim al growth conditions herbicides may become particularly toxic for cultural plants. Plants inhibited by herbicides show distinctly higher boron contents than that of untreated samples. 
  Reference    Z. Naturforsch. 44c, 781—786 (1989); received April 24 1989 
  Published    1989 
  Keywords    Silicon, Boron, Plant G row th, H erbicide, Spinach 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0781.pdf 
 Identifier    ZNC-1989-44c-0781 
 Volume    44 
6Author    Wolfgang Schmidt, Ulrich Schreiber, Wolfgang UrbachRequires cookie*
 Abstract    The effects of short-time fumigation (0-60 min) of intact spinach leaves with S0 2 (2 ppm) on the photosynthetic apparatus were investigated. A rather high S0 2 concentration was applied to monitor immediate effects on the fluorescence behaviour with the influence of repair processes or secondary types of damage being minimized. Three different types of in vivo chlorophyll fluores-cence measurements were used: Rapid induction kinetics (Kautsky effect), slow induction kinetics with repetitive application of saturation pulses (saturation pulse method), and decay kinetics following a single turnover saturating flash. The slow induction kinetics with repetitive application of saturation pulses reacts in the most sensitive way indicating a primary damage at the level of the enzymatic reactions of the Calvin cycle. It is suggested that stromal acidification upon S0 2 uptake interferes with light activation of Calvin cycle enzymes. With longer fumigation times also damage at the level of photosystem II becomes apparent: A decrease in variable fluorescence yield reflects a lowering of photosystem II quantum yield, and the slowing down of fluorescence relaxation kinetics reveals an effect on the secondary electron transport from Q A to Q B . The detrimental effects of S0 2 depend to a great extent on the application of light during fumigation. Besides a light requirement for S0 2 uptake by stomata opening also the possibility of photoinhibitory damage is discussed. The susceptibility of leaves to photoinhibition may increase with a lowering of Calvin cycle activity by S0 2 . 
  Reference    Z. Naturforsch. 43c, 269—274 (1988); received December 7 1987 
  Published    1988 
  Keywords    Chlorophyll Fluorescence, S0 2, Spinach, Intact Leaves, Calvin Cycle 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0269.pdf 
 Identifier    ZNC-1988-43c-0269 
 Volume    43