1 | Author | Hans-Joachim Soli, Ellen Olschewski | Requires cookie* |
Title | Photoaffinity Labeling o f Spinach Thylakoids and Cytochrome ^ ![]() | ||
Abstract | /-C o m p le x by the Hydrophobic Reagent 3-(Trifluorom ethyl)-3-(/n-[125I]iodophenyl)-diazirine W alter O ettm eier, In isolated spinach thylakoids the hydrophobic photo reactive probe 3-(trifluoromethyl)-3-(m-[I25I]iodophenyl-diazirine almost exclusively labels the photosystem I reac tion center and the light-harvesting chlorophyll alb protein. In isolated cytochrome b6//-complex, all four components of the complex get labeled, but to a different extent. The amount of labeling in the protein components is correlated to the number of polypeptide segments embedded in the membrane system. | ||
Reference | Z. Naturforsch. 40c, 454 (1985); received February 25 1985 | ||
Published | 1985 | ||
Keywords | Photoaffinity Label, Carbene Reagent, Spinach Thylakoids, Cytochrome b j f -complex, Membrane Proteins | ||
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