| 2 | Author
| T. Hom As Brandlm, Hugo Scheer, W. Olfhart Rüdiger | Requires cookie* | | Title
| Chromophore Content and Molar Absorptivity of Phytochrome in the Pr Form  | | | Abstract
| Quantitative absorption spectra in the visible and UV region were recorded for denaturated phytochrome (P r) and phytochromobilin peptides in comparison with native Pr. The method was tested with C-phycocyanin from Spirulina platensis. Based on known molar absorptivities for denaturated phycocyanin and suitable model compounds, and on the ratio ^niüve/^delrS = 2.9, ^native was determined to be 1 0 2 0 0 0 m _1 cm-1 for one phycocyanobilin chromophore in native phycocyanin. Likewise, N a t iv e was calculated to be 19000 M -1 cm-1. The corresponding ratios for Pr Were ^native/^denatured = 3*4 and A native/-^degraded = 3.7, t h l S yielded ^native = 109000 tO 118000 and eSUfcve = 36000 M _1 cm-1 for phytochromobilin in native P r. This value corresponds to one phytochromobilin per small phytochrome (60000 D) if data from the literature are corrected for the content o f colorless proteins. The latter has been asessed from (i) the purity index (A2g0/A665) and (ii) the contribution o f the phytochromobilin chromophore at 280 nm as derived from model compounds. | | |
Reference
| Z. Naturforsch. 36c, 431 (1981); received February 20 1981 | | |
Published
| 1981 | | |
Keywords
| Absorption, Spectroscopy, A-Dihydrobilindiones, Isophorcabilin, Phycobiliproteins, Phytochrome, Phytochromobilin-Peptides | | |
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| default:Reihe_C/36/ZNC-1981-36c-0431.pdf | | | Identifier
| ZNC-1981-36c-0431 | | | Volume
| 36 | |
3 | Author
| Hans-Peter Köst, Eva Benedikt, Edmund Cmiel, Siegfried Schneider | Requires cookie* | | Title
| 5N-Coprobiliverdin, a New Model Chromophore  | | | Abstract
| The synthesis of l5N-labeled coprobiliverdin III, a bile pigm ent with four conjugated pyrrole nuclei and four carboxylic acid side chains, is described. C oprobiliverdin is structurally characterized by chemical and various spectroscopic m ethods (e.g. chrom ic acid degradation, UV-vis-, IR-, mass spectroscopy). The results of 15N N M R studies reveal the potential value of this compound as a probe for structural interactions in biliproteins and as a reference for the future investigation of natural bile pigments deriving | | |
Reference
| Z. Naturforsch. 38c, 943 (1983); received July 7 1983 | | |
Published
| 1983 | | |
Keywords
| Natural Bile Pigments, Coprobiliverdin III, Model C hrom ophore, Spectroscopy, MO-Calculations | | |
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| default:Reihe_C/38/ZNC-1983-38c-0943.pdf | | | Identifier
| ZNC-1983-38c-0943 | | | Volume
| 38 | |
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