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1Author    John Wilcockson, Dietrich WernerRequires cookie*
 Title    On the DNA Content of the Bacteroids of Rhizobium japonicum  
 Abstract    The DNA content of bacteroids from large nodules of soybean plants infected with Rhizobium japonicum strain 61 -A -101 was found to be 1.20 x 10~14 g per cell. Bacteroids from smaller nodules had slightly less DNA as did the stationary phase, free-living cells; both giving a value of 0.92 x 10-14 g per cell. In comparing these data with those of other workers it was found that there is little evidence to support suggestions that bacteroids possess anything less than a full genetic complement of DNA and that some misinterpretation of older published data has occurred. 
  Reference    Z. Naturforsch. 34c, 793 (1979); received June 15 1979 
  Published    1979 
  Keywords    DNA Content, Nodules, Bacteroids, Rhizobium, Soybean 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0793.pdf 
 Identifier    ZNC-1979-34c-0793 
 Volume    34 
2Author    Joachim Leube, Hans GrisebachRequires cookie*
 Title    Further Studies on Induction of Enzymes of Phytoalexin Synthesis in Soybean and Cultured Soybean Cells  
 Abstract    The glucan elicitor from cell walls o f the fungal pathogen, Phytophthora m egasperm a f. sp. glycinea, caused a decrease in activity o f 3-hydroxy-3-m ethylglutaryl coenzym e A reductase in wounded soybean cotyledons, whereas wounding alone led to an increase in the activity o f this enzyme. A decrease o f HM G -CoA reductase activity after elicitor treatment was also found in soybean hypocotyls and soybean cell cultures. In contrast to the activity o f H M G -CoA reductase, the activity o f dim ethylallylpyrophosphate: 3,6a,9-trihydroxypterocarpan dimethylallyltransferase increases after elicitor challenge o f soy­ bean cell cultures and after inoculation o f soybean hypocotyls (cv A m so y 7 1) with m ycelium o f either race 1 (incom patible) or race 3 (com patible) o f P. megasperma. 
  Reference    Z. Naturforsch. 38c, 730 (1983); received June 23 1983 
  Published    1983 
  Keywords    Phytoalexins, Soybean, Soybean Cell Cultures, Enzyme-Induction 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0730.pdf 
 Identifier    ZNC-1983-38c-0730 
 Volume    38 
3Author    Christine Gareis, Christiane Rivero, Ingolf Schuphan, Burkhard SchmidtRequires cookie*
 Title    Plant Metabolism of Xenobiotics. Comparison of the Metabolism of 3.4-Dichloroaniline in Soybean Excised Leaves and Soybean Cell Suspension Cultures  
 Abstract    Excised leaves and cell suspension cultures of soybean (Glycine m ax L.) were incubated with [UL-14C]-3,4-dichloroaniline. The compound was almost completely metabolized after 48 h in both systems; it was apparent that the major detoxification pathways present in the excised leaves were also present in the cultured cells. Besides considerable amounts of insoluble resi­ dues, the N-glucosyl and the N-malonyl conjugates of 3,4-dichloroaniline, and a yet unknown metabolite was formed in the excised leaves; tentatively the latter was identified with the 6'-0-malonylester of N-glucosyl-3,4-dichloroaniline. The cell suspension cultures produced predominantly the N-malonyl conjugate, besides negligible amounts of the N-glucosyl conju­ gate, and insoluble residues; the majority of the N-malonyl compound was excreted into the medium. It was shown, that the metabolism of 3,4-dichloroaniline in excised leaves and cell suspension cultures of soybean was directed towards different end products: the excised leaves were able to make extensive use of cell wall structures as a deposit for xenobiotic bound resi­ dues, resembling plants in this respect; lack of these structures in the cultured soybean cells resulted in a soluble end product, namely the N-malonyl conjugate. 
  Reference    Z. Naturforsch. 47c, 823—829 (1992); received August 5 1992 3. 
  Published    1992 
  Keywords    4-Dichloroaniline, Metabolism, Soybean, Cell Culture, Excised Leaves 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0823.pdf 
 Identifier    ZNC-1992-47c-0823 
 Volume    47 
4Author    Kerstin Kosch3, Andreas Jacobi3, Martin Parniskeb, Dietrich Werner3, Peter Müller3Requires cookie*
 Title    Bradyrhizobium japonicum Exopolysaccharide Mutant is Determined by the Genotype of the Host Plant  
 Abstract    The deletion mutant Bradyrhizobium japonicum AP22 produces a structurally altered exo­ polysaccharide. The nodulation of two cultivars each of Glycine max and Glycine soja, and cultivars of Macroptilium atropurpureum and Vigna radiata, infected with this mutant was examined in order to analyze the role of the exopolysaccharide in the infection process of plants with a determinate nodule type. All host plants analyzed exhibited delayed nodulation and formed fewer nodules per plant. The extent of the impairments depended on the geno­ type of the host plant. Morphological studies confirmed these results. In V radiata later steps in nodule development proceeded without further disturbances, whereas with G. soja PI 407287 minor changes were detected. In contrast, the inoculation of G. soja PI 468397 and M. atropurpureum lead to the formation of nodules most of which were not infected by Bradyrhizobium japonicum AP22 (In f-). However, on M. atropurpureum at least some effec­ tive nitrogen-fixing nodules developed. Such nodules did not emerge from G. soja PI 468397. Inf~ nodules were arrested in an early stage of nodule development, and symptoms of plant defense responses were observed. 
  Reference    Z. Naturforsch. 49c, 727 (1994); received August 3 1994 
  Published    1994 
  Keywords    Bradyrhizobium, Exopolysaccharides, Nodulation, Determinate Nodule Type, Soybean 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0727.pdf 
 Identifier    ZNC-1994-49c-0727 
 Volume    49 
5Author    U. Zähringer1, E. Schaller2, H. Grisebach1Requires cookie*
 Title    Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean  
 Abstract    Using HPLC on a partisil column (5 nm) it was possible to separate two reaction products from an enzyme incubation o f dimethylallylpyrophosphate and 3,6a,9-trihydtoxypterocarpan with a particulate fraction from elicitor-treated soybean cotyledons (Harosoy 63) or soybean cell cultures. These two products were identified by MS and !H-NMR analysis as 2-and 4-dimethylallyltrihy-droxypterocarpan. Both compounds also occur as natural products in elicitor-treated soybean cotyledons and to a smaller extent in soybean cell cultures. Introduction of the dimethylallyl substituent into trihydroxypterocarpan increases the fungitoxicity against Cladosporium cucume-rinum. Upon treatment with alkali, solutions o f 3,6a,9-trihydroxypterocarpans turn to an intense red colour with Amax around 530 nm. One of the products from 2-DMA-THP could be isolated by reversed phase HPLC. According to its mass spectrum a quinoid structure is proposed for this red pigment. 
  Reference    Z. Naturforsch. 36c, 234—241 (1981); received December 81980 
  Published    1981 
  Keywords    Phytoalexins, Pterocarpans, Dimethylallyl Transferase, Soybean, Glycine max L, Red Pigment 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0234.pdf 
 Identifier    ZNC-1981-36c-0234 
 Volume    36 
6Author    P. Moesta, U. Seydel, B. Lindner, H. G. RisebachRequires cookie*
 Title    Detection of Glyceollin on the Cellular Level in Infected Soybean by Laser Microprobe Mass Analysis  
 Abstract    Laser microprobe mass analysis was explored as a m ethod for the detection at the cellular level of the phytoalexin glyceollin in soybean cotyledons infected with an incom patible race of Phy­ tophthora megasperma f. sp. glycinea. For LAMMA® analysis 10 |im freeze microtome sections which were freeze-dried on copper grids were used. The LAM M A spectrum of glyceollin (isomer I) shows a characteristic peak at m /e = 321 which can be attributed to M -O H +. This peak was also present in the spectra of infected regions from a cotyledon b u t was completely absent in the spec­ tra of uninfected tissue. One hundred and fifty LAM M A spectra were taken along a line per­ pendicular to the border line o f infection. A steep rise in glyceollin content toward the infected area was observed. This is the First tim e th at such highly localized glyceollin accumulation has been shown at the cellular level. The results show th at LAM M A analysis is suitable for the de­ tection of organic molecules in biological tissues w ith high lateral resolution. 
  Reference    Z. Naturforsch. 37c, 748—751 (1982); received May 19 1982 
  Published    1982 
  Keywords    Glyceollin, Phytoalexins, Soybean, Glycine m ax, L, LAM MA 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0748.pdf 
 Identifier    ZNC-1982-37c-0748 
 Volume    37