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1Author    Dieter StrackRequires cookie*
 Title    Sinapine as a Supply of Choline for the Biosynthesis of Phosphatidylcholine in Raphanus sativus Seedlings  
 Abstract    Biosynthesis o f phosphatidylcholine in young seedlings o f Raphanus sativus is supplied with choline from degradation of the seed constituent sinapine (sinapoylcholine). This conclusion has been deduced from the following results: (1) Raphanus sativus seedlings accumulate approx. 70 nmol phosphatidylcholine which may be relevant for the consumption of choline, liberated during hydrolysis of approx. 130 nmol sinapine. (2) [14C]choline and [14C]ethanolamine, taken up by germinating Raphanus seeds, are exclu­ sively consumed in the biosynthesis o f phosphatidylcholine and phosphatidylethanolamine, respectively. (3) Pulse-chase experiments with seedlings at different germination stages show a reduced [14C]choline incorporation into phosphatidylcholine at the time when degradation o f sinapine occurs, obviously as a result of an isotope dilution by an increase o f the endogenous choline pool. (4) After [14C]choline pulse to immature seeds, during the process o f seed maturation most of the activity taken up is incorporated into accumulating sinapine and approx. 50% compared to this into phosphatidylcholine. During seedling development the quantity o f labelled sinapine rapidly decreases as a result of sinapine degradation with a concomitant label increase in free choline, phosphorylcholine, and phosphatidylcholine. Approx. 50% of the choline liberated from sinapine, is consumed in the biosynthesis of phosphatidylcholine. High performance liquid chromatographic analyses of phosphatidylcholine during Raphanus germination revealed that this phospholipid might be a metabolically active compound. Changes in the absorptivity of this compound at 210 nm indicate changes in the degree of unsaturation in the fatty acyl groups. 
  Reference    Z. Naturforsch. 36c, 215—221 (1981); received December 231980 
  Published    1981 
  Keywords    Sinapine, Phosphatidylcholine, Phenylpropanoids, Phospholipids, Raphanus, Brassicaceae 
  Similar Items    Find
 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0215.pdf 
 Identifier    ZNC-1981-36c-0215 
 Volume    36