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1980 (1)
1979 (1)
1Author    M. Ichael Linscheid, Zentralbereich Forschung, Entwicklung Der, Bayer Ag, D. Leverkusen, Ieter StrackRequires cookie*
 Title    The Structures of Sinapic Acid Esters and Their Metabolism in Cotyledons of Raphanus sativus  
 Abstract    Five sinapic acid esters were isolated from cotyledons of Raphanus sativus and have been iden­ tified with the aid of electron impact (El)-, field desorption (FD)-mass spectrometry and NMR spectroscopy: 6,3'-disinapoylsucrose, sinapoylcholine (sinapine), 6 -sinapoylglucoraphenine, 1 -si­ napoylglucose, and sinapoylmalate. Three of these derivatives are metabolically related in the se­ quence sinapoylcholine -» • 1 -sinapoylglucose — ► sinapoylmalate. 
  Reference    Z. Naturforsch. 35c, 907—914 (1980); received August 5 1980 
  Published    1980 
  Keywords    Raphanus sativus, Brassicaceae, Sinapic Acid Esters, Phenylpropanoids, High Performance Liquid Chromatography 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0907.pdf 
 Identifier    ZNC-1980-35c-0907 
 Volume    35 
2Author    G. Erhild, N. Urm, Ann, D. Ieter StrackRequires cookie*
 Title    Sinapine Esterase I. Characterization of Sinapine Esterase from Cotyledons of Raphanus sativus  
 Abstract    From cotyledons o f Raphanus sativus (red radish) an esterase activity which catalyzes the hy­ drolysis o f sinapine into sinapic acid and choline has been isolated. The enzyme, which has a near absolute specificity, is not analogous with any esterase described in the literature. The reaction has a pH optim um o f 8.5 and the apparent K m is 1.95 x 10~5 m. The enzyme is relatively insensi­ tive to both physostigm ine (eserine) {K x = 1.73 x 10-4 m) and neostigm ine (A'i = 2 .1 3 x 10-4 m). Diisopropyl fluorophosphate (D F P) showed no inhibition and diethyl /?-nitrophenylphosphate (E 600) only a slight inhibitory effect at 10-5 m, respectively. Choline (10~2 m) was inhibitory but acetylcholine (1 0 -2 m) stimulated the enzyme activity. 
  Reference    Z. Naturforsch. 34c, 715—720 (1979); received June 11 1979 
  Published    1979 
  Keywords    Esterase, Sinapine, Sinapic Acid Esters, Raphanus, Brassicaceae, High-Performance Liquid Chro­ matography (HPLC) 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0715.pdf 
 Identifier    ZNC-1979-34c-0715 
 Volume    34