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'Silene' in keywords Facet   section ZfN Section C  [X]
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1984 (1)
1981 (2)
1Author    Jan Van Brederode, RiaK. Am, Ps-H EinsbroekRequires cookie*
 Title    Structure and Biosynthesis o f Vitexin 2"-0-Xyloside in Silene alba  
 Abstract    In petals o f Silene alba plants o f Armenian populations, vitexin 2"-0-xyloside was demonstrated to be present. The enzyme, catalyzing the transfer of the xylose moiety of UDP-xylose to vitexin, has its optimal activity at pH 7.8. Mg2+ and Mn2+ stimulated the reaction. The K m values were 2 x 10-5 M for vitexin and 3 x 10-5 M for UDP-xylose. 
  Reference    Z. Naturforsch. 36c, 484 (1981); received February 20 1981 
  Published    1981 
  Keywords    Silene, Caryophyllaceae, Biosynthesis, Vitexin 2"-0-Xyloside, Xylosyltransferase 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0484_n.pdf 
 Identifier    ZNC-1981-36c-0484_n 
 Volume    36 
2Author    Jan Van Brederode, Ria Kamps-HeinsbroekRequires cookie*
 Title    Methylation of 3',4' Di-O H C-Glycosyl- flavones in Silene  
 Abstract    In green parts of Silene plants of the genotype g lR two methylated flavonoids were found: isoscoparin and isosco-parin 2"-0-rhamnoside. An enzyme has been demonstrated to catalyze the transfer of the methyl moiety of S-adenosyl methionine to iso-orientin and iso-orientin 2"-0-rhamnosi-de. Maximal activity takes place at pH 8.0 -8 .2 . Of the me­ tal ions Mn2+, Mg2+, Ca2+, Co2+, Zn2+ and Hg2+, only Co2+ stimulated the reaction at conc. > 2 m M . For the methyla­ tion o f isoorientin the K m values were 4 x 10-6 M for S-ade-nosyl methionine and 0.32 x 10~3 m for iso-orientin. When isoorientin 2"-0-rhamnoside was used as substrate the K m values were 5 x 10"6 m for S-adenosylmethionine and 7 x 10-6 M for iso-orientin 2"-0-rhamnoside. 
  Reference    Z. Naturforsch. 36c, 486 (1981); received February 20 1981 
  Published    1981 
  Keywords    Silene, Caryophyllaceae, Methylation, Biosynthesis, Iso-scoparin 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0486_n.pdf 
 Identifier    ZNC-1981-36c-0486_n 
 Volume    36 
3Author    J. M. Steyns, O. M. Astenbroek, *. @bullet, G. Van Nigtevecht, J. Van BrederodeRequires cookie*
 Title    07g and D6a: Two Flavone Glycosylating Genes in Silene, which are Only Expressed in Cotyledons and Rosette Leaves  
 Abstract    Two further loci can be added to the three already known (g , g l and f g) in Silene controlling the 7 -0 and 2"-0-glycosylation o f the C -glycosylflavone isovitexin (6-C -glucosylapigenin). These loci, 0 7 g for the 7-O-galactosylation and D6a for the 2"-0-arabinosylation, appear only to be expressed in cotyledons and rosette leaves and control the biosynthesis o f isovitexin 7 -0 -galactoside and isovitexin 7-0-galactose 2"-0-arabinosid e in these parts o f plants in which the recessive alleles o f the loci g, g l and f g are hom ozygous (Steyns et al. [9]). The possibility that locus g, whose dominant alleles gG and g X control the 7-0-glu cosylation and the 7-0-xylosylation o f isovitexin respectively, controls the synthesis o f isovitexin-7-O -galactoside was excluded by demonstrating the presence o f isovitexin-7-O -galactoside and the 7-0-galactosyltransferase catalyzing its biosynthesis in addition to isovitexin-7-O -glucoside and the 7-0-glucosyltransferase in cotyledons o f gGgG seedlings. The synthesis o f the 2"-0-arabinoside is neither controlled by locus gl, whose dom inant alleles glR and glA control the 2"-0-rham nosylation and 2"-0-arabinosylation o f isovitexin respectively, nor by locus fg, whose dom inant allele Fg controls the biosynthesis o f isovitexin 2"-0-glucoside. This was shown by the presence o f isovitexin-7-O -galactose 2"-0-arabinosid e and the 2"-0-arabinosyltransferase catalyzing the synthesis o f the 2"-0-arabinosid e in addition to isovitexin-7-O-galactose 2"-0-rham noside in cotyledons o f g lR g lR seedlings and by the presence o f isovitexin-7-O-galactose 2"-0-arabinoside and the 2"-0-arabinosyltransferase in addition to isovitexin-7-O-galactose 2"-0-glucoside and the 2"-0-glucosyltransferase in cotyledons o f FgFg seedlings. 
  Reference    Z. Naturforsch. 39c, 568—5 (1984); received January 26/M arch 16 1984 
  Published    1984 
  Keywords    Silene, Flavone Glycosylation Genes, O 7g: Isovitexin 7-O -G alactoside, D6a: Isovitexin 2"-0-A rabinoside, Ontogeny 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0568.pdf 
 Identifier    ZNC-1984-39c-0568 
 Volume    39