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1978 (1)
1975 (1)
1Author    M. Volm, G. MerzRequires cookie*
 Title    Einfluß von Cytoplasma-und Serumfaktoren auf den [3H]Thymidintriphosphat-Einbau isolierter Leberzellkerne Effect of Cytoplasmic and Serum Factors on the Uptake of [3H]Thymidine- triphosphate (TTP) by Isolated Nuclei of Liver Cells  
 Abstract    The actions of cytoplasm (ultracentrifuged supernatant 105 000 x g) and of serum on the DNA synthesis (uptake of [3H ]T T P) by isolated nuclei of liver cells were studied. Cytoplasm from liver cells obtained at various intervals after partial hepatectomy revealed a variable effect only with isolated nuclei of liver cells from partially hepatectomized rats. Cytoplasm obtained from liver cells eight hours after p artial hepatectomy failed to show the usual inhibitory effect. Presum ably the inhibitory substances norm ally present in cytoplasm are inactivated within a certain time after partial hepatectomy. Serum of partially hepatectomized anim als enhanced the uptake of [3H ]T T P by nuclei of normal liver cells and those from partially hepatectom ized animals. The stim ulating effect was present only in the serum obtained between four and twelve hours after partial hepatectomy. It remains unclear whether the effects described are growth factors specific for the liver. 
  Reference    (Z. Naturforsch. 30c, 107 [1975]; eingegangen am 10. Oktober 1974) 
  Published    1975 
  Keywords    DNA-Synthesis, Isolated Liver N uclei, Cytoplasm, Serum, P artial Hepatectomy 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0107.pdf 
 Identifier    ZNC-1975-30c-0107 
 Volume    30 
2Author    R. Strasser, M. VolmRequires cookie*
 Title    Untersuchungen über spezifische Einflüsse des Serums und Lebercytoplasmas auf den [3H]TTP-Einbau isolierter Leberzellkerne Studies on the Specific Effects of Serum and Cytoplasm of the Liver on the [3H]TTP-Incorporation of Isolated Liver Nuclei  
 Abstract    Previous authors have described that liver cytoplasm and serum from partially hepatectomized rats stimulate the DNA synthesis of isolated nuclei of liver cells. The aim of our investigation was to prove whether such stimulating effects are part of a specific regulation of growth. The sera and the cytoplasm supernatant 105 000 Xg) were isolated at several intervals after partial hepatectomy and laparotomy and were tested on isolated nuclei of liver cells from adult rats partially hepatectomized 20 hours before. The supernatants isolated at 8 and 16 hours after both partial hepatectomy and laparotomy have a significant stimulatory effect on the [3H]TTP-uptake by nuclei. The presence of serum (10%) causes a general elevation (75%) of the [3H]TTP-uptake by isolated nuclei. The sera isolated 4 to 12 hours after operation from both partially hepatectomized and laparotomized rats have an additional stimulatory effect on the [3H] TTP-uptake synthes of nuclei. In no case did the sera and liver cytoplasm of partially hepatectomized rats have a significantly different and therefore "specific" effect on the nuclear DNA synthesis. Additional experiments showed that frozen and thawed nuclei of liver cells respond only to the nutritive power but not to the stimulatory effects of the sera. Thus, thawed nuclei cannot be used for growth investigations. 
  Reference    Z. Naturforsch. 33c, 219 (1978); eingegangen am 5. September 1977/25. Januar 1978 
  Published    1978 
  Keywords    Isolated Liver Nuclei, Partial Hepatectomy, [3H] TTP-Incorporation, Serum, Cytoplasm 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0219.pdf 
 Identifier    ZNC-1978-33c-0219 
 Volume    33