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1992 (1)
1974 (1)
1Author    N. N. Osborne, V. Neuhoff, B. E. LeonardRequires cookie*
 Title      
 Abstract    The effects of a number of phenylethylamines on the metabolism of brain biogenic amines have been studied with the aim of differentiating neurochemi-cally between those drugs having a stimulant profile from those which are hallucinogenic 1_5. The pre­ sent study was undertaken to see if some of these drugs had a similar effect on the metabolism of serotonin in the oesophageal ganglia of the snail so that an assessment could be made of the possible usefulness of this in vitro preparation in the initial 
  Reference    (Z. Naturforsch. 29c, 189 [1974]; received December 12. 1973) 
  Published    1974 
  Keywords    Amphetamine, Amino Acids, Serotonin, Helix pomatia 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0189_n.pdf 
 Identifier    ZNC-1974-29c-0189_n 
 Volume    29 
2Author    J. Berlinab, I. N. Kuzovkinac, C. Rügenhagenab, L. Fecker3, U. C. Om, M. Andeur3, V. W. RaybRequires cookie*
 Title    Hairy Root Cultures of Peganum harmala II. Characterization of Cell Lines and Effect of Culture Conditions on the Accumulation of ß-Carboline Alkaloids and Serotonin  
 Abstract    Hairy root cultures o f Peganum harmala established by genetic transformation with A gro­ bacterium rhizogenes wild type strains A 4, 15834 or carrying the binary vector pLTCgus 1, re­ spectively, were analyzed with respect to their tryptophan-derived m etabolites. Beside the pre­ viously detected ß-carboline alkaloids harmine, harmol, harmaline and harmalol all hairy root cultures were found to contain the ß-carboline glucoside ruine and serotonin. The appearance o f the root cultures and the levels o f ß-carboline alkaloids were greatly affected by the nitrogen source o f the medium. In the presence o f higher levels o f ammonium ions (as in MS medium) the roots were short and thick, while in B50 medium (low ammonium) they were thin and long. Ammonium ions had a negative effect on the levels o f ß-carbolines. The best root cul­ tures accumulated on B50 1 .5 -2 % ß-carbolines on a dry mass basis and in the presence o f 6% sucrose ca. 10 mg ß-carbolines/L x d were formed. In the presence o f phytohorm ones root cul­ tures were converted into low producing suspension cultures, from which the highly produc­ tive roots were reinduced on phytohormone-free medium. 
  Reference    Z. Naturforsch. 47c, 222 (1992); received July 29/Novem ber 11 1991 
  Published    1992 
  Keywords    Peganum harmala, R oot Cultures, Transformation, ß-Carboline Alkaloids, Serotonin 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0222.pdf 
 Identifier    ZNC-1992-47c-0222 
 Volume    47