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2000 (1)
1999 (1)
1Author    K.-K Lee, K.-C Yii, T.-I Yang, H.-I Hong, P.-C LiuRequires cookie*
 Title    Vibrio carchariae after Growth on Various Media  
 Abstract    Protease and virulence of the extracellular products (EC P) of Vibrio carchariae strain Em I82KL, a causative agent of gastroenteritis in E pinephelus coioides, cultured on different media were studied. The bacteria grown on peptone agar, nutrient agar or brain heart infu­ sion agar produced higher protease activities than that grown on tryptic soy agar (T SA) in terms of protein content. The addition of ethylenediamine di(o-hydroxyphenylacetic acid) or horse serum in TSA enhanced the ECP protease production while the addition of grouper serum apparently reduced the enzyme activity indicating the presence of protease inhibitor(s) in the fish serum. Furthermore, the use of grouper meat or peptone as a single nutrient source remarkably enhanced the production of ECP protease. Adding proteinaceous materials from animal sources (horse serum, grouper meat or peptone) on agar manifested higher ECP protease activity than that from plant source (T SA), indicating the intestine of carnivorous groupers might favour the existence, survival or infection of the bacterium. The protease was a metal-chelator-sensitive serine protease since it was inhibited by 3,4-dichloroisocoumarin and phenylmethanesulfonyl fluoride while about 80% of its activity inhibited by chelating agents (ethylene-diaminetetraacetic acid and ethylene glycol-bis(y3-amino-ethylether) N,N,N',N'-tetraacetic acid). The ECP obtained from each medium was not lethal to the groupers suggesting that the bacterium is low virulent. As grouper is carnivorous, therefore, the role of the protease played in the fish intestine probably is competing for nutrients and/ or associated with the cause of edema leading to gastroenteritis. 
  Reference    Z. Naturforsch. 54c, 383—3 (1999); received January 4 1999 
  Published    1999 
  Keywords    Serine Protease, Vibrio carchariae, Media, Grouper, Virulence 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0383.pdf 
 Identifier    ZNC-1999-54c-0383 
 Volume    54 
2Author    Ferng-Ruey Chen, Ping-Chung Liu, Kuo-Kau LeeRequires cookie*
 Title    Lethal Attribute of Serine Protease Secreted by Vibrio alginolyticus Strains in Kuruma Prawn Penaeus japonicus  
 Abstract    Toxicity of the extracellular products (E C P) and the lethal attribute of serine protease secreted by five pathogenic Vibrio alginolyticus strains from various sources in kuruma prawn Penaeus japonicus were studied. The E C P s of organisms originally isolated from diseased kuruma prawn or small abalone Haliotis diversicolor supertexta were more lethal (L D 50 value of 0.48 or 0.41 ^ig protein/g prawn) than those from diseased tiger prawn P. m onodon, yellow-fin porgy Acanthopagrus latus or horse m ackerel (L D 50 value of 0 .9 8 -1 .1 7 ^ig protein/g prawn). All the EC Ps manifested strong, weak and no activities against gelatin, sheep eryth­ rocytes and chitin, respectively. In immunodiffusion tests using rabbit antiserum to a purified 33 kDa serine protease of strain Swy against E C P of each tested strain produced one single precipitation band in each treatm ent. Furtherm ore, the serine protease was suggested to be the dominant protease secreted by V. alginolyticus strains tested since the majority of enzy­ matic activity of the respective E C P was inhibited by phenylmethanesulfonyl fluoride (PM SF). A higher inhibition of serine protease activity by PM SF resulted in lower mortality rate of the EC Ps injected into the prawns suggesting that the protease is one of the major lethal factor(s) secreted by V alginolyticus. 
  Reference    Z. Naturforsch. 55c, 94—9 (2000); received Septem ber 9/Novem ber 8 1999 
  Published    2000 
  Keywords    Extracellular Products, Lethality, Penaeus japonicus, Serine Protease, Vibrio alginolyticus 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0094.pdf 
 Identifier    ZNC-2000-55c-0094 
 Volume    55