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'Sequence Analysis' in keywords Facet   section ZfN Section C:Volume 045  [X]
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1990 (3)
1Author    G. Ewald, C. Wiessner, H. MichelRequires cookie*
 Title    Sequence Analysis of Four Atrazine-Resistant Mutants from Rhodopseudomonas viridis  
 Abstract    Four atrazine-resistant mutants from the purple bacterium Rhodopseudom onas viridis were isolated. Sequence analysis revealed three different mutant strains carrying mutations in the herbicide-binding pocket: i) M AV 2: L 212-G lu —* Lys, ii) M AV 3: L216-Phe —» Ser and iii) M AV 4 = MAV 5: L217-Arg His, L220-Val Leu. Except M AV 3 all Rps. viridis mutants are different from those selected by their resistance towards the closely related triazine terbutryn. 
  Reference    Z. Naturforsch. 45c, 459 (1990); received December 9 1989 
  Published    1990 
  Keywords    Herbicide-Resistant Mutants, Atrazine, Photosynthetic Reaction Center, Sequence Analysis 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0459.pdf 
 Identifier    ZNC-1990-45c-0459 
 Volume    45 
2Author    Dirk Naber, Udo Johanningmeier, JackJ S Van Rensen, D. N., J.J S V RRequires cookie*
 Title    A Rapid Method for Partial mRNA and DNA Sequence Analysis of the Photosystem II psbA Gene  
 Abstract    Single amino acid substitutions in the D 1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between hel­ ices IV and V of the protein. The increasing number of herbicide-resistant organisms necessi­ tates development of a rapid methodology to characterize deviations from the wildtype se­ quence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D 1. These procedures involve the isolation and amplification of D N A and RN A and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-re-sistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D 1 protein, is found in the resistant plant. 
  Reference    Z. Naturforsch. 45c, 418—422 (1990); received November 3 1989 
  Published    1990 
  Keywords    Triazine Resistance, Sequence Analysis, Polymerase Chain Reaction, Chenopodium album 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0418.pdf 
 Identifier    ZNC-1990-45c-0418 
 Volume    45 
3Author    MichaelD. Partis, A.F R, R.Udolf GrimmRequires cookie*
 Title    Computer Analysis of Phytochrome Sequences from Five Species: Implications for the Mechanism of Action  
 Abstract    The amino acid sequences o f phytochrom e from A vena sativa, O ryza sativa, Curcurbita pepo, Pisum sativum and Arabidopsis thaliana have been analyzed with a variety o f computer programs, with a view to identifying areas o f the protein which contribute to the properties o f this photoreceptor. A region at the C-terminus has been shown to be amphiphilic, and by ana­ logy with surface-seeking peptides, may be responsible for interaction o f phytochrom e with lipid bilayers. Possible targeting sequences in phytochrom es have been identified, including a series o f four basic residues which correspond to those responsible for transport o f nuclear-located proteins. Sites capable o f post-translational m odification have been found in m onocot sequences, but not in dicot sequences. Areas o f the phytochrom e molecule which are exposed on the surface o f the portein, and which are therefore capable o f interaction with other cellular macromolecules, have been identified. A nalogies with other biliproteins have been used to de­ fine minimum chromophore-protein interactions. Possible enzymic activities associated with phytochromes have been discussed with respect to local amino acid sequence similarity with enzymes. 
  Reference    Z. Naturforsch. 45c, 987—9 (1990); received April 12 1990 
  Published    1990 
  Keywords    Phytochrome, Sequence Analysis, Amphiphilic Peptides, M embrane Interaction, Targeting Sequences 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0987.pdf 
 Identifier    ZNC-1990-45c-0987 
 Volume    45