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1994 (1)
1Author    Jutta Thiem, Wolfgang BarzRequires cookie*
 Title    Photoautotrophic Chenopodium rubrum Cell Suspension Cultures Resistant against Photosynthesis-Inhibiting Herbicides I. Selection and Characterization  
 Abstract    For establishing metribuzin-resistant, photoautotrophic Chenopodium rubrum cell cul­ tures plated cells, callus cultures or suspension cultures were subjected to selection proce­ dures. The most effective procedure was the stepwise increase in the concentration of the herbicide from 0.01 ^im to 10 ^im in suspension cultures, which resulted in the isolation of eight different metribuzin-resistant photoautotrophic cell lines. Conjugation metabolism or a decrease in the uptake and translocation of the selective agent were not responsible for resist­ ance, which was stable in the absence of the inhibitor over numerous growth cycles. Meas­ urements of the photosynthetic electron transport, analyses of fluorescence induction kinetics and determination of the binding properties of 14C-labelled metribuzin to isolated thylakoids indicated that resistance of the cell lines is based on an alteration in the photosystem II her­ bicide-binding protein (D 1 protein). RFLP analysis of the psbA gene of the eight resistant cell lines demonstrated that none of them possess an amino acid exchange in position 264 of the D 1 protein leading to altered herbicide-binding properties. 
  Reference    Z. Naturforsch. 49c, 186—194 (1994); received June 25/December 161993 
  Published    1994 
  Keywords    Herbicide Resistance, Selection Mechanism, Photoautotrophic Cell Culture, Chenopodium rubrum 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0186.pdf 
 Identifier    ZNC-1994-49c-0186 
 Volume    49