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1994 (1)
1983 (1)
1Author    A. Brennicke, V. H. Em LebenRequires cookie*
 Title    Highly Repetitive Satellite DNA  
 Abstract    The primary sequence of the prom inent Cucumis melo satellite DNA is presented. The cloned Hind Ill-repeat unit has a length of 352 bp and a GC-content o f 56%. W ithin this sequence an inverted repeat of 9 bp, including a 286 bp loop, and one direct repeat are shown. Several open reading frames are possible following the sequence in both directions whose significance is not yet clear. The sequence has been com pared with other highly repetitive DNA sequences of Sinapis alba and Scilla sibirica. The genomic satellite DNA exhibits an unusual behaviour in actinomycin D-CsCl gradients in comparison to ribosomal D NA 
  Reference    Z. Naturforsch. 38c, 1062—1065 (1983); received October 28 1983 
  Published    1983 
  Keywords    DNA Sequence, Satellite DNA, Cucumis melo 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-1062_n.pdf 
 Identifier    ZNC-1983-38c-1062_n 
 Volume    38 
2Author    ThiloC. Fischer, Sabine Groner, Ulrike Zentgraf, Vera HemlebenRequires cookie*
 Title    Evidence for Nucleosomal Phasing and a Novel Protein Specifically Binding to Cucumber Satellite DNA  
 Abstract    The nucleosomal organization and the protein-binding capability of highly repeated and methylated satellite DNA of cucumber (Cucumis sativus L.), comprising approx. 30% of the genome, were analyzed. Nucleosomal core DNA from satellite type I was prepared after micrococcal nuclease digestion of chromatin and sequenced. Most of the core sequences ob­ tained could be grouped in two main (A and B) and two minor groups (C and D) indicating a specific and complex phasing of nucleosomes on this satellite DNA. In vitro, gel retarda­ tion assays with cloned satellite DNA repeats (types I-IV) demonstrated a specific binding of nuclear proteins. These specific binding effects are also obtained with genomic, in vivo methylated and sequence heterogeneous (1 to 10% diversity) satellite type I DNA. For the first time in plants, a satellite DNA-binding protein with an apparent molecular weight of 14 kDa (SAT 14) was identified. 
  Reference    Z. Naturforsch. 49c, 79—86 (1994); received October 28/November 121993 
  Published    1994 
  Keywords    Cucumis sativus, DNA Methylation, DNA-Protein Interaction, Nucleosomal Phasing, Satellite DNA 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0079.pdf 
 Identifier    ZNC-1994-49c-0079 
 Volume    49