| | 1 | Author
| Bemd Schulz-Harder, Claudia Kiicherer | Requires cookie* | | | Title
| in Saccharomyces cerevisiae  | | | | Abstract
| The ribosomal ribonuclease of yeast is induced after a withdrawal of glucose. Cycloheximide inhibits the synthesis of the enzyme under conditions of induction but antimycin shows no effect. Hence, the increase of the ribonuclease activity during growth of yeast is due to newly synthesized enzyme, and the induction does not depend on respiration. | | | |
Reference
| Z. Naturforsch. 35c, 168—170 (1980); received February 13/September 5 1979 | | | |
Published
| 1980 | | | |
Keywords
| Yeast, Ribosomes, Ribonuclease, Cycloheximide, Antimycin | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0168_n.pdf | | | | Identifier
| ZNC-1980-35c-0168_n | | | | Volume
| 35 | |
| 2 | Author
| H. Seki, W. Schnabel | Requires cookie* | | | Title
| On the Free Radical-Induced Aggregation of Ribonuclease — A Pulse Radiolysis Study Using the Light Scattering Detection Method | | | | Abstract
| The aggregation of bovine ribonuclease (RNase) induced by either -OH or BrJradicals has been studied. These radicals, which were generated by pulse radiolysis of aqueous solutions with 16 MeV electrons, readily attack the enzyme thus producing RNase-radicals which subsequently combine. At initial radical concentrations low enough (^ 0.1 radical per enzyme molecule) to prevent multimerizations other than dimerization, rate constants for the latter process were determined from the increase o f the light scattering intensity after the pulse: 2 k2 = (2.2 ± 0.3) 10® 1/mol s (Brj initiated dimerization) and (5.4 ± 0.4) 10® 1/mol s (O H initiated dimerization). The G-values for dimerization are: 1.3 (BrJ) and 0.85 (O H). Transient optical absorption measurements revealed the existence o f phenoxyl radicals of_tyrosine (TyrO-) that decayed with a rate constant o f 2 k2 (total) = (5.5 ± 0.7) 10® 1/mol s (Brj-case). The difference between k2 and k2 (total) presumably indicates the occurrence o f disproportionation. | | | |
Reference
| Z. Naturforsch. 37c, 63—69 (1982); received September 161981 | | | |
Published
| 1982 | | | |
Keywords
| Free Radical-Induced Aggregation, Ribonuclease, Light Scattering Detection Method | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0063.pdf | | | | Identifier
| ZNC-1982-37c-0063 | | | | Volume
| 37 | |
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