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'Ribonuclease' in keywords Facet   Publication Year 1982  [X]
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1Author    H. Seki, W. SchnabelRequires cookie*
 Title    On the Free Radical-Induced Aggregation of Ribonuclease — A Pulse Radiolysis Study Using the Light Scattering Detection Method  
 Abstract    The aggregation of bovine ribonuclease (RNase) induced by either -OH or BrJradicals has been studied. These radicals, which were generated by pulse radiolysis of aqueous solutions with 16 MeV electrons, readily attack the enzyme thus producing RNase-radicals which subsequently combine. At initial radical concentrations low enough (^ 0.1 radical per enzyme molecule) to prevent multimerizations other than dimerization, rate constants for the latter process were determined from the increase o f the light scattering intensity after the pulse: 2 k2 = (2.2 ± 0.3) 10® 1/mol s (Brj initiated dimerization) and (5.4 ± 0.4) 10® 1/mol s (O H initiated dimerization). The G-values for dimerization are: 1.3 (BrJ) and 0.85 (O H). Transient optical absorption measurements revealed the existence o f phenoxyl radicals of_tyrosine (TyrO-) that decayed with a rate constant o f 2 k2 (total) = (5.5 ± 0.7) 10® 1/mol s (Brj-case). The difference between k2 and k2 (total) presumably indicates the occurrence o f disproportionation. 
  Reference    Z. Naturforsch. 37c, 63—69 (1982); received September 161981 
  Published    1982 
  Keywords    Free Radical-Induced Aggregation, Ribonuclease, Light Scattering Detection Method 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0063.pdf 
 Identifier    ZNC-1982-37c-0063 
 Volume    37