| 1 | Author
| Hiroko Ito, K. Aran, RhodaElison Hirsch, SeymourSteven Brody | Requires cookie* | | Title
| Stability and Regeneration of Rhodopsin Absorption Spectra at an Air-Water Interface  | | | Abstract
| Stability and regenerability of the absorption spectrum of CTAB solubilized rhodopsin at an air-water interface is studied. The spectral properties of rhodopsin films in the dark are stable more than 150 minutes. When rhodopsin is bleached (40 — 50%) and maintained in highly com pressed films recovery of the spectrum is observed. The recovery is 57% to 100% of the pigment present before irradiation. At low surface pressure or if the films are expanded after irradiation there is no observable recovery. | | |
Reference
| Z. Naturforsch. 33c, 317 (1978); received March 28 1978 | | |
Published
| 1978 | | |
Keywords
| Vision, Rhodopsin, Monolayers, Membranes, Photobiology | | |
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| default:Reihe_C/33/ZNC-1978-33c-0317.pdf | | | Identifier
| ZNC-1978-33c-0317 | | | Volume
| 33 | |
2 | Author
| A. Ndreas Schleicher, KlausPeter Hofmann | Requires cookie* | | Title
| Proton Uptake by Light Induced Interaction between Rhodopsin and G-Protein  | | | Abstract
| The light-induced proton uptake of rod outer segment disc membranes has been investigated in the absence and presence of G-protein. Proton uptake was measured as the alkalisation of the suspending medium using a pH electrode and/or the indicator dye bromocresol purple. It was found that besides the known proton uptake of photolysed rhodopsin additional uptake of one proton accompanies formation of the complex between rhodopsin and G-protein. No measurable proton uptake was found under conditions of rapid redissociation of the complex indicating an only transient protonation during its lifetime. Proton uptake was the same in washed membra nes recombined with G-protein and in ordinarily stacked rod outer segments. The additional proton uptake reported here is not due to enhanced metarhodopsin II. | | |
Reference
| Z. Naturforsch. 40c, 400—405 (1985); received January 14/February 25 1985 | | |
Published
| 1985 | | |
Keywords
| Photoreceptor, Protein-Protein Interaction, GTP-Binding Protein, Rhodopsin | | |
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| default:Reihe_C/40/ZNC-1985-40c-0400.pdf | | | Identifier
| ZNC-1985-40c-0400 | | | Volume
| 40 | |
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