| | 1 | Author
| Michaela Preuß, Jobst-H Einrich Klemme | Requires cookie* | | | Title
| Purification and Characterization of a Dissimilatory Nitrite Reductase from the Phototrophic Bacterium Rhodopseudomonas palustris  | | | | Abstract
| A dissimilatory nitrite reductase from the facultatively phototrophic bacterium , Rhodopseudo monas palustris strain la l was studied. A basic level o f the enzyme (1 0 -5 0 m U /m g protein) was measured in dark, aerated and anaerobic, photosynthetic cultures. A m arked derepression of enzyme synthesis occurred under conditions of oxygen lim itation (2 0 0 -3 0 0 m U /m g protein). The addition of nitrite (or nitrate) to the culture m edium had only a slight effect on the maximal nitrite reductase titer o f cells. The enzyme was purified from photosynthetically grown cells by precipitation with am m onium sulfate, gel filtration through Sepharose 6 B and repeated chromatography on DE 52-cellulose. As estimated by gel filtration, the nitrite reductase had a molecular weight o f about 120 000 + 12 000 and yielded only one band (mol. wt. o f about 68 000 + 7000) in SDS-gel electrophoresis. The isoelectric point o f the enzyme was at pH 5.1. Nitric oxide (NO) was identified as the reaction product of nitrite reduction. The enzyme also exhibited cytochrome c-oxidase activity and was active with chemically reduced viologen dyes, FMN and cytochrome c as electron donors. Highly purified nitrite reductase preparations con tained 10mol% of a c-type cytochrome. Trace metal analyses indicated the presence o f Cu in the enzyme. Consistent with the detection of Cu was the finding that the C u-chelator, diethyl-dithiocarbamate, strongly inhibited the nitrite reductase. | | | |
Reference
| Z. Naturforsch. 38c, 933—938 (1983); received July 11/Septem ber 8 1983 | | | |
Published
| 1983 | | | |
Keywords
| N itrite Reductase, N itrite D issimilation, Phototrophic Bacterium, Rhodopseudomonas palustris | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0933.pdf | | | | Identifier
| ZNC-1983-38c-0933 | | | | Volume
| 38 | |
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