| 1 | Author | Kassem Alef, Hans-Joachim Burkardt, Hans-Joachim Horstmann, WalterG. Zumft | Requires cookie* |
| Title | Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseudomonas palustris1  | ||
| Abstract | The phototrophic bacterium Rhodopseudomonas palustris assimilated ammonium via glutamine synthetase and glutamate synthase. Diazotrophic and ammonium-grown cells had high levels of both enzymes, whereas enzymes o f alternative assimilatory pathways were absent or had only low activities. Glutamine synthetase was purified to electrophoretic homogeneity within three steps by dye-ligand and ion exchange chromatography. Electron microscopy revealed a dodecameric molecular entity which was in accordance with parameters derived from electrophoretic techniques. The molecular weight of the enzyme monomer was 55800; that o f the dodecamer 670000. The amino acid composition o f R. palustris glutamine synthetase was determined and compared by a statistical method with other known enzyme compositions from prokaryotic and eukaryotic origins. | ||
| Reference | Z. Naturforsch. 36c, 246—254 (1981); received December 231980 | ||
| Published | 1981 | ||
| Keywords | Rhodopseudomonas palustris, Glutamine Synthetase, Dye-Ligand Chromatography, Nitrogen Fixation, Ammonium Assimilation | ||
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