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'Rhodobacter capsulatus' in keywords Facet   section ZfN Section C  [X]
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1991 (1)
1990 (1)
1Author    Z. NaturforschRequires cookie*
 Title    N o Correlation between Plasmid Content and Ability to Reduce N itrate in W ild-Type Strains o f Rhodobacter capsulatus  
 Abstract    Patterns o f endogenous plasmids and nitrate reduc­ tase activities were analyzed in the phototrophic bacte­ rium Rhodobacter (Rb.) capsulatus. From 10 strains in­ vestigated (including a UV-induced plasmidless nit~ mu­ tant), 4 were unable to grow photosynthetically with nitrate as N-source and lacked nitrate reductase activity (nit strains). Irrespective o f the nit phenotype, all 
  Reference    Z. Naturforsch. 46c, 703 (1991); received May 10 1991 
  Published    1991 
  Keywords    Nitrate, Plasmid, Photosynthetic Bacteria, Rhodobacter capsulatus 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0703_n.pdf 
 Identifier    ZNC-1991-46c-0703_n 
 Volume    46 
2Author    Stefan Stevanovic, Günther Jung, JürgP. RosenbuschRequires cookie*
 Abstract    The major outer membrane protein of Rhodobacter capsulatus 37b4 (capsule-free) was iso­ lated. Strong porin-activity was observed after reconstitution into artificial lipid bilayer mem­ branes with a single channel conductance of 3.15 nS in Im KC1. The porin migrated as a broad, single band (M T above 90,000) on sodium dodecyl sulfate polyacrylamide gel electro­ phoresis and dissociated into a single species of polypeptides (M r 36,000) on treatment with EDTA (10 m M at 30 °C, 20 min) or by heating (100 °C, 5 min). Analytical ultracentrifugation studies demonstrated the native porin to be a trimer. The monomers chromatofocused as a single, sharp peak on fast performance liquid chromatography and only one band, corre­ sponding to an isoelectric point of about 4.0, was obtained on isoelectric focusing. Gas-phase sequencing of the 23 N-terminal residues revealed G lu-V al-Lys-Leu-Ser-G ly-A sp-Ala-Arg-M et-G ly-V al-M et-T yr-A sn-Gly-A sp-Asp-X-A sn-Phe-Ser-Ser. 
  Reference    Z. Naturforsch. 45c, 576—582 (1990); received February 19 1990 
  Published    1990 
  Keywords    Rhodobacter capsulatus, Cell Wall, Outer Membrane, Porin, Oligomeric Structure 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0576.pdf 
 Identifier    ZNC-1990-45c-0576 
 Volume    45