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1982 (1)
1979 (1)
1Author    John Wilcockson, Dietrich WernerRequires cookie*
 Title    On the DNA Content of the Bacteroids of Rhizobium japonicum  
 Abstract    The DNA content of bacteroids from large nodules of soybean plants infected with Rhizobium japonicum strain 61 -A -101 was found to be 1.20 x 10~14 g per cell. Bacteroids from smaller nodules had slightly less DNA as did the stationary phase, free-living cells; both giving a value of 0.92 x 10-14 g per cell. In comparing these data with those of other workers it was found that there is little evidence to support suggestions that bacteroids possess anything less than a full genetic complement of DNA and that some misinterpretation of older published data has occurred. 
  Reference    Z. Naturforsch. 34c, 793 (1979); received June 15 1979 
  Published    1979 
  Keywords    DNA Content, Nodules, Bacteroids, Rhizobium, Soybean 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0793.pdf 
 Identifier    ZNC-1979-34c-0793 
 Volume    34 
2Author    Peter Müller, DietrichW. ErnerRequires cookie*
 Title    Rhizobium japonicum  
 Abstract    Alanine dehydrogenase (E.C. 1.4.1.1.) from nitrogenase repressed free living cells o f Rhizobium japonicum 61-A-101 was purified 370 fold to a specific activity o f 30.4 (imol pyruvate • min-1 • mg protein-1. The same enzyme from effective bacteroids from nodules o f Glycine max var. Mandarin, infected with the same strain was purified 150 fold to a specific activity o f 35 units. The enzyme from both preparations was identical in the molecular weight o f about 168 kD with four identical subunits of 42 kD. The alanine dehydrogenase is, therefore, different from the same enzyme from Bacillus subtilis (molecular weight 228 kD) and from Anabaena cylindrica (molec­ ular weight 270 kD). The K m data for the enzyme from Rhizobium japonicum are: 4.7 mmol/1 for NH+, 0.68 mmol/1 for pyruvate and 44 nmol/1 for NADH. Specific activity o f the enzyme in total cell extracts from eight other strains of Rhizobium japonicum (3 effective strains, 5 ineffective strains) was only 20 to 30% o f the activity with strain 61-A -101. N o correlation between alanine dehydrogenase activity and nitrogenase activity in these other eight strains was observed. The function of alanine dehydrogenase in Rhizobium japonicum in ammonium assimilation and cell wall differentiation is discussed. 
  Reference    Z. Naturforsch. 37c, 927 (1982); received May 19 1982 
  Published    1982 
  Keywords    Rhizobium, Bacteroid Differentiation, Alanine Dehydrogenase, Glutamate Dehydrogenase, Ammonium Assimilation 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0927.pdf 
 Identifier    ZNC-1982-37c-0927 
 Volume    37