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1984 (1)
1979 (1)
1Author    H. Berndt, D. Wölfle, H. Bahl, A. SteinbiichlRequires cookie*
 Title    Evidence for Carbon Monoxide Insensitive Respiration in the Aerobic Nitrogen Fixing Bacteria Azotobacter vinelandii OP and Xanthobacter autotrophicus GZ 29  
 Abstract    In intact cells of the non-gummy Azotobacter vinelandii OP CO-insensitivity of the respiratory activity increased with decreasing dissolved oxygen tension in the bacterial suspension. Upon changing from low to high aeration conditions the CO-sensitive respiration was restored. Mea­ surement of the oxidative activity of small particles of A. vinelandii OP with N A D H , ascorbate-DCPIP and ascorbate-TMPD as substrate in the presence and absence of CO indicated that the CO-insensitive site is probably identical with cytochrome , which preferably is reduced by elec­ trons from ascorbate-DCPIP. Small particles of the gum producing A. vinelandii NCIB 8660 ap­ peared to be more sensitive towards CO with ascorbate DCPIP than the non-gummy A. vinelandii OP. In small particles the CO-insensitive DCPIP oxidase was present irrespective of the dissolved oxygen tension during cell growth. In intact cells, however, CO-insensitivity was only expressed at low dissolved oxygen tension when electrons are directed to cytochrome a ^ o in the branched re­ spiratory chain. Intact cells of Xanthobacter autotrophicus G Z 29 exhibited a similar CO-insensi-tive respiration as A. vinelandii OP. 
  Reference    Z. Naturforsch. 34c, 576—581 (1979); received February 26/April 5 1979 
  Published    1979 
  Keywords    Azotobacter vinelandii, Xanthobacter autotrophicus, Carbon Monoxide, Respiratory Chain 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0576.pdf 
 Identifier    ZNC-1979-34c-0576 
 Volume    34 
2Author    AugustoF. Garcia, G.Erhart DrewsRequires cookie*
 Title    Properties of Membrane Fractions Prepared by Chromatophore-Liposome Fusion  
 Abstract    Intracytoplasmic membrane vesicles (chromatophores) isolated from Rhodopseudomonas capsulata cells were fused with liposom es by a pH transition procedure. Vesicles o f lower density and higher lipid contents and larger diam eter than chrom atophores were obtained. Similar results were observed by Ca2+ induced fusion and by the freeze-thawing method. Respiratory and light-induced electron transport were m easured in chrom atophores and fused vesicles. Light-induced reaction center bleaching was observed in all types o f vesicles, whereas repiratory electron transport was substantially dim inished by lipid incorporation. U biquinone 10 restored to some extent respiratory electron transport and oxidative phosphorylation and it m odified the photophosphorylation kinetics under continuous light. Electrochrom ic carotenoid band-shift and the 9-aminoacridine fluorescence quenching indicate that the capacity o f the fused vesicles to maintain an electrochemical proton gradient has not been substantially dim inished. From the kinetics o f 9-aminoacridine quenching an increased K+-perm eability seem s to be apparent. 
  Reference    Z. Naturforsch. 39c, 1112—1119 (1984); received July 23 1984 
  Published    1984 
  Keywords    Photosynthetic Apparatus, Respiratory Chain, Rhodopseudom onas capsulata, M embrane Fusion, Liposomes, Photophosphorylation 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1112.pdf 
 Identifier    ZNC-1984-39c-1112 
 Volume    39