| | 1 | Author
| T. V. Rao, U. C. Biswal | Requires cookie* | | | Title
| Isolation and Respiratory A ssay o f Earthworm Body W all M itochondria  | | | | Abstract
| Out o f several media tested, KC1 m edium contain ing BSA was found to be most suitable for isolation o f functionally efficient mitochondria from earthworms. Respiration and oxidative phosphorylation in isolated mitochondria are demonstrated in the presence o f cyto chrome c and BSA in reaction medium. Introduction — The details o f the respiratory m etabolism in earthworm are yet to be established. There are only a few contradictory reports on the nature o f its respiratory enzyme components [1], W hile, cytochrome oxidase, suc-cinoxidase, succinate dehydrogenase were identified in earthworm tissue homogenates, the presence o f Kreb's cycle enzymes could not be demonstrated. Very little information is available on earthworm m itochondria except one report where it is isolated in 0.44 M m annitol [2]. Mitochondria from invertebrates like insects and crustaceans are isolated and characterized [3, 4]. But such methods cannot be extended to earthworms as it is known that conditions for isolation and assay o f m itochondria are highly system specific [5]. | | | |
Reference
| Z. Naturforsch. 39c, 853 (1984); received January 23 1984 | | | |
Published
| 1984 | | | |
Keywords
| Earthworm Mitochondria, Isolation, Respiratory Assay, Metabolism, Oxidative Phosphorylation | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0853_n.pdf | | | | Identifier
| ZNC-1984-39c-0853_n | | | | Volume
| 39 | |
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