| 1 | Author | Frank Seela, Wolfgang Wesemann | Requires cookie* |
| Title | Trägergebundene Serotoninderivate und Affinitätschromatographie synaptischer Membranproteine Resin Linked Derivatives of Serotonin and Affinity Chromatography of Proteins Isolated from Synaptic Membranes  | ||
| Abstract | An affinity resin for the isolation of the serotonin receptor from nerve ending membranes was prepared by coupling tryptamine, 5-methoxy-tryptamine or serotonin to CNBr-activated Sepharose 4 B. The tryptamine derivatives, N-6-(aminohexyl)-tryptamines, were obtained by subsequent con densation of the corresponding indole derivatives with oxalyl chloride and t-aminocaproamide and reduction with L iA lH 4 . The 6-aminohexyl-group was used as spacer to link covalently the primary amino-group of tryptamine to agarose. — An extract of membrane proteins isolated from rat brain was resolved on this affinity resin into two fractions as compared with only one fraction using unsubstituted Sepharose 4 B. | ||
| Reference | (Z. Naturforsch. 29c, 248—256 [1974]; eingegangen am 2. Januar/15. Februar 1974) | ||
| Published | 1974 | ||
| Keywords | Affinity Chromatography, Receptor, Resin Linked Serotonin, Synaptic Membrane Proteins | ||
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